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Comparison of biochemical characterizations with PCR ampli fi cation in identi fi cation of Malassezia species isolated from pityriasis versicolor and healthy volunteers |
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| รหัสดีโอไอ | |
| Creator | 1. Proawpilart Intayot 2. Sirida Youngchim |
| Title | Comparison of biochemical characterizations with PCR ampli fi cation in identi fi cation of Malassezia species isolated from pityriasis versicolor and healthy volunteers |
| Publisher | Faculty of Medicine |
| Publication Year | 2559 |
| Journal Title | Chiang Mai Medical Journal |
| Journal Vol. | 55 |
| Journal No. | 1(Suppl) |
| Page no. | 31-43 |
| Keyword | โรคเกลื้อน,เชื่อ Malassezia,สปีซีส์,PCR,การทดสอบทางชีวเคมี |
| ISSN | 0125-5983 |
| Abstract | The aim of this research was to determine the correlation between biochemical features and a molecular method in the identi fi cation of Malassezia spp. To achieve this study, 173 isolates of Malassezia spp. were collected from 8 patients with pityriasis versicolor and 165 healthy volunteers. Differentiation of Malassezia spp. was performed by using biochemical test and PCR ampli fi cation. The agreement between biochemical features and PCR ampli fi cation was found 73.41% (127/173) in the identi fi cation of M. sympodialis, M. furfur, M. dermatis and M. slooffi ae. In particular for M. furfur and M. sympodialis, the higher percentages of the agreement between these two methods were found 81.94 and 77.63%, respectively. In addition, 46 isolates of Malassezia spp. were differentiated by PCR ampli fi cation only and then con fi rmed by genomic sequencing. Six species of Malassezia including M. sympodialis (36.95%), M. furfur (28.26%), M. dermatis (21.74%), M. slooffi ae (4.35%), M. globosa (4.35%), and M. japonica (4.35%), were detected. These fi ndings suggest that PCR ampli fi cation can be used as a potential tool in identi fi cation of Malassezia spp. when compared with the biochemical method. However, the biochemical studies are suitable for the presumptive identi fi cation of Malassezia species where molecular methodologies are not available. |
