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Clinical application of molecular testing for the diagnosis of primary myelo fi brosis clinicopathologically mimicking chronic myelogenous leukemia: a case report |
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| รหัสดีโอไอ | |
| Creator | 1. Ekarat Rattarittamrong 2. Ratchanu Tongphung 3. Adisak Tantiworawit 4. Chatree Chai-Adisaksopha 5. Sasinee Hantrakool 6. Lalita Norrasetthada 7. Suree Lekawanvijit |
| Title | Clinical application of molecular testing for the diagnosis of primary myelo fi brosis clinicopathologically mimicking chronic myelogenous leukemia: a case report |
| Publisher | Faculty of Medicine |
| Publication Year | 2558 |
| Journal Title | Chiang Mai Medical Journal |
| Journal Vol. | 54 |
| Journal No. | 1 |
| Page no. | 39-45 |
| Keyword | primary myelofi brosis,chronic myelogenous leukemia,RT-PCR for BCR-ABL1,RQ-PCR for BCR-ABL1,JAK2 V617F mutation analysis |
| ISSN | 0125-5983 |
| Abstract | Background Chronic myelogenous leukemia (CML) and primary myelo fi brosis (PMF) are both myeloproliferative neoplasms that have overlapped clinical manifestations, but their prognosis and treatment are different. Besides conventional chromosome analysis, molecular testing is required for the correct diagnosis in some patients.Objective To present a case report that applied molecular techniques to diagnose PMF in a patient with CML-like presentation.Results The patient was a 66 year-old male who presented with splenomegaly and marked leukocytosis with left shift. CML was suspected, but chromosome analysis did not demonstrate a Philadelphia chromosome. Reverse transcription- polymerase chain reaction (RT-PCR) and real-time quantitative PCR (RQ-PCR) also were negative for the identi fi cation of BCR-ABL1. Analysis of positive JAK2 V617F mutations as well as increased reticulin fi bers in a bone marrow biopsy supported the diagnosis of PMF.Conclusion Molecular diagnostic techniques are required for the diagnosis of MPNs, especially in patients with overlapping clinical manifestations of CML and PMF |
