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Cytotoxicity of the Calcium Alginate/ N,O-carboxymethylchitosan Hemostatic Sponge on Primary Human Gingival Fibroblasts |
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| รหัสดีโอไอ | |
| Creator | Marnisa Sricholpech |
| Title | Cytotoxicity of the Calcium Alginate/ N,O-carboxymethylchitosan Hemostatic Sponge on Primary Human Gingival Fibroblasts |
| Contributor | Tanawat Mahatchariyapong, Kaltimas Phaewpala, Wasana Kosorn, Wanida Janvikul, Kitwat Kitrueangphatchara |
| Publisher | Faculty of Dentistry, Chiang Mai University |
| Publication Year | 2565 |
| Journal Title | Chiang Mai Dental Journal |
| Journal Vol. | 43 |
| Journal No. | 1 |
| Page no. | 19-28 |
| Keyword | CA/NOCC sponge, calcium ion, cell morphology, cell proliferation |
| URL Website | http://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/index |
| Website title | Chiang Mai Dental Journal |
| ISSN | 2773-921X |
| Abstract | To date, chitosan-based hemostatic agents have gained increasing interest from their biocompatibility, inexpensiveness and hemostatic capability, especially in coagulopathic conditions. We have developed a functionally improved Calcium Alginate/N,O-carboxymethylchitosan (CA/NOCC) hemostatic sponge, shown to be biocompatible and biodegradable.Objectives: To ensure its safe use with the gingival tissue, this study aimed to evaluate the cytotoxicity of CA/NOCC sponge on primary human gingival fibroblasts (GFs).Methods: Human GFs were cultured with or without the CA/NOCC sponge. Cell morphology was assessed by scanning electron microscopy. Cell viability and proliferation were determined by MTT assays. The levels of Ca2+ released into the culture medium were also measured.Results: Gingival fibroblasts cultured with the CA/NOCC sponge demonstrated lowered cell density, and significant ultrastructural changes of the cell membrane, by forming numerous blebs and fibrils. From MTT assays, approximately 30% decrease in the proliferation rate was observed. Moreover, the levels of Ca2+, up to 4.6 mM, were detected in the medium of GFs cultured with the CA/NOCC sponge.Conclusions: It could be implicated that the cytopathic effects on the morphology and proliferative ability of GFs may result from the high level of Ca2+ released from the CA/NOCC hemostatic sponge. |
