|
Biological Effects of Pulp Capping Material Containing Fluocinolone Acetonide on Human Dental Pulp Cells |
|---|---|
| รหัสดีโอไอ | |
| Creator | Phumisak Louwakul |
| Title | Biological Effects of Pulp Capping Material Containing Fluocinolone Acetonide on Human Dental Pulp Cells |
| Contributor | Tadkamol Krongbaramee, Wilawan Louwakul, Veera Lertchirakarn |
| Publisher | Faculty of Dentistry, Chiang Mai University |
| Publication Year | 2564 |
| Journal Title | Chiang Mai Dental Journal |
| Journal Vol. | 42 |
| Journal No. | 3 |
| Page no. | 85-94 |
| Keyword | dental pulp, fluocinolone acetonide, inflammation, in vitro |
| URL Website | http://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/index |
| Website title | Chiang Mai Dental Journal |
| ISSN | 2773-921X |
| Abstract | Objectives: The aim of this study was to test some biological properties of pulp capping material containing fluocinolone acetonide (PCFA).Materials and Methods: The PCFA is a hard setting calcium hydroxide cement containing 50 mmol/L of fluocinolone acetonide (FA). Cytotoxicity and cell proliferation were evaluated by MTT assay. RT-PCR and Western blotting were used to study the effects of PCFA on RNA (dentin sialophosphoprotein: DSPP) and protein (dentin sialoprotein: DSP) synthesis. Anti-inflammatory effect of PCFA was determined by analysis of cyclooxygenase 2 (COX-2) expression. Dycal® was used as control.Results: The diluted conditioned media from PCFA slightly increased cell proliferation, significantly increased DSPP expression and decreased COX-2 expression (p < .05).Conclusions: In term of biologic properties, the PCFA may promote cell proliferation, mineralization and decrease pre-existing inflammation in human dental pulp cells. It may be considered as an alternative pulp capping material in the treatment of inflamed dental pulp. |
