The Use of Ozone for Controlling European House Dust Mite,Dermatophagoides pteronyssinus (Trouessart)
รหัสดีโอไอ
Creator Jarongsak Pumnuan
Title The Use of Ozone for Controlling European House Dust Mite,Dermatophagoides pteronyssinus (Trouessart)
Contributor Ammorn Insung and Teerapong Wangapai
Publisher King Mongkut's Institute of Technology Ladkrabang
Publication Year 2563
Journal Title Current Applied Science and Technology
Journal Vol. 20
Journal No. 3
Page no. 420-428
Keyword Acari, Pyroglyphidae, Dermatophagoides pteronyssinis, ELISA, fumigation, allergies, ozone, Der p1
URL Website https://www.tci-thaijo.org/index.php/cast
Website title https://www.tci-thaijo.org/index.php
ISSN 2586-9396
Abstract Hypersensitivity allergies are mostly caused by house dust mite (HDM), Dermatophagoidespteronyssinus (Trouessart). Common control methods for HDM include disposal of bedding setsand fabrics, vacuuming, washing, using plant extracts and applying chemicals. Hence, theapplication of ozone is a new alternative way to control HDM. The objectives of this experimentwere to determine the efficacy of ozone as an HDM killer and to evaluate the allergic levels thatremained after the application of ozone. The fumigation method was performed in a laboratory usingozone at the concentrations of 20, 30, 40 and 50 mg l-1 in glass chambers (1 m3) at 1, 2 and 3 hfumigation intervals. The mortality percentages of HDM were observed at 24 h after the treatment.The allergic levels appearing in supernatants were analyzed by an enzyme-linked immunosorbentassay (ELISA) method. Ozone fumigations of 30 mg l-1 concentration for 3 h completely killed HDM. In addition, ozone fumigations of ? 40 mg l-1 for 3 h reduced the amount of allergen by >50%, which was a significantly higher reduction than seen at 20-30 mg l-1(35.8-45.8%). The study suggests the ozone fumigation at 30 mg l-1 concentration treated for at least 3 h, 40 mg l-1for 2 h, or 50 mg l-1 for 1 h could be used as a new alternative method to control HDM
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