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A New Method To Detect IDH1 Synonymous SNP 105 C >T rs11554137 |
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| รหัสดีโอไอ | |
| Creator | Yasser B.M. Ali |
| Title | A New Method To Detect IDH1 Synonymous SNP 105 C >T rs11554137 |
| Contributor | Ahmed F.K. Mohamed,Gamal T. Ebid and Adel A. Guirgis |
| Publisher | King Mongkut's Institute of Technology Ladkrabang |
| Publication Year | 2561 |
| Journal Title | Current Applied Science and Technology |
| Journal Vol. | 18 |
| Journal No. | 3 |
| Page no. | 192 |
| Keyword | IDH, SNP, RFLP, AML, Glioma |
| URL Website | https://www.tci-thaijo.org/index.php/cast |
| Website title | https://www.tci-thaijo.org/index.php |
| ISSN | 2586-9396 |
| Abstract | Alteration of cellular metabolism is one of the oncogenetic mechanisms. Furthermore, the defect of Isocitrate dehydrogenase (IDH) enzymes lead to abnormal cellular metabolism. Mutation in the IDH1 gene is common in glioblastoma multiforme (GMB), glioma, and acute myeloid leukemia (AML). The IDH1 single nucleotide polymorphism (SNP) in codon 105 C>T (rs11554137) is a prognostic factor in patients with glioma and AML. Currently, the most common technique used for IDH1 SNP 105 C>T detection is the sequencing technique that expensive and time-consuming. We designed a new method through the Restriction fragment length polymorphism (RFLP). Ninty Eight unrelated healthy volunteers comprising of 49 male, and 49 female which was randomly selected. Their ages ranged from 1 to 64 years with a median age of 33 years. Polymerase chain reaction (PCR) and RsaI fragmented IDH1 SNP105 C >T were identified in 16.3% of our cases. So we conclude that we may use the PCR-RFLP assay instead. |
