|
Involvement of red blood cell on calcium oxalate crystal growth and aggregation in vitro |
|---|---|
| รหัสดีโอไอ | |
| Creator | Mohamed Shahid |
| Title | Involvement of red blood cell on calcium oxalate crystal growth and aggregation in vitro |
| Contributor | Nattaya Sae-ung, Porntip Pinlaor, Patcharee Boonsiri, Wichien Sirithanaphol, Vitoon Prasogwattana, Ratree Tavichakorntrakool |
| Publisher | Faculty of Associated Medical Sciences, Khon Kaen University, Thailand |
| Publication Year | 2564 |
| Journal Title | Archives of Allied Health Sciences |
| Journal Vol. | 33 |
| Journal No. | 1 |
| Page no. | 62-69 |
| Keyword | Red blood cell, Calcium oxalate, Crystal growth, Crystal aggregation |
| URL Website | https://he01.tci-thaijo.org/index.php/ams/about |
| Website title | Archives of Allied Health Sciences (Arch AHS) |
| ISSN | 2730-1990 |
| Abstract | Cell membranes and their components may play an important role in stone formation. Hematuria is one of the most common manifestations in kidney disease. We therefore extensively investigate the involvement of intact red blood cell (iRBC) and red blood cell membrane fragments (fRBC) on CaOx crystal growth and aggregation. Intact RBC and fRBC were prepared from healthy blood samples. Calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) crystals were investigated for crystal growth and aggregation in the condition without or with iRBC or fRBC. Crystal growth and aggregation were analyzed from crystal area and the number of crystal aggregation, respectively, and also confrmed by using spectrophotometric oxalate depletion assay and calcium oxalate crystal aggregation-sedimentation assay, respectively. The results showed that only COM crystal with fRBC was signifcantly increased the crystal area and the number of crystal aggregation as compared to control conditions (p-value = 0.035 and p-value = 0.011, respectively) while COD crystals did not have any signifcant change to the crystal area or the number of crystal aggregates with both types of RBC. These data indicated that fRBC might promote the growth and aggregation of COM crystals. However, the molecular mechanism involvement of fRBC with COM crystals still needs to be studied. |
