|
Screening of fungi isolated from damaged plant materials for the production of lignocellulolytic enzymes with decolorizing ability |
|---|---|
| รหัสดีโอไอ | |
| Creator | Paripok Phitsuwan |
| Title | Screening of fungi isolated from damaged plant materials for the production of lignocellulolytic enzymes with decolorizing ability |
| Contributor | Damkerng Bundidamorn, Lakha Salaiphet, Taweerat Vichitsoonthonkul, Khanok Ratanakhanokchai |
| Publisher | Asia-Pacific Journal of Science and Technology |
| Publication Year | 2564 |
| Journal Title | Asia-Pacific Journal of Science and Technology |
| Journal Vol. | 26 |
| Journal No. | 3 |
| Page no. | 10 |
| Keyword | Curvularia sp., Fusarium sp., Decolorization, Food waste, Lignocelluloytic enzyme, Phanerochaete sp., Solid-state fermentation |
| URL Website | https://www.tci-thaijo.org/index.php/APST |
| Website title | https://so01.tci-thaijo.org/index.php/APST/article/view/249397 |
| ISSN | 2539-6293 |
| Abstract | Lignocellulolytic fungi are the major sources of enzymes, mainly cellulase, xylanase, and oxidative enzymes. In this study, three fungal species, identified as strains TV-1, PLS-21#7, and PSA-3, were isolated from damaged areas of infected plants. Based on morphological observation, the species were classified into Phanerochaete sp. TV-1, Curvularia sp. PLS21#7, and Fusarium sp. PSA-3, respectively. The fungi were grown on agar media containing carboxymethyl cellulose and Beechwood xylan, due to which clear zones surrounded the colonies on both agar media. The results indicate that strains Phanerochaete sp. TV-1, Curvularia sp. PLS21#7, and Fusarium sp. PSA-3 have cellulolytic and xylanolytic activities. The fungal isolates were grown on ground cassava pulp and durian peel at the temperature of 25 ? for ten days to determine their growth ability and enzyme production profile. Unlike durian peel, cassava pulp induced cellulase and xylanase synthesis for the three fungal strains. Although the crude enzyme of Phanerochaete sp. TV-1, grown on cassava pulp, showed higher cellulase and xylanase activities than those of Curvularia sp. PLS21#7, and Fusarium sp. PSA-3, the decolorizing ability against Indigo carmine was not different among the three strains (p-value>0.05), showing around 40-60% decolorization within three days. Our study demonstrates that the new Phanerochaete sp. TV-1, Curvularia sp. PLS21#7, and Fusarium sp. PSA-3 strains can be used as hosts for enzyme production in low-cost media. These enzymes can be further applied in biomass saccharification or treatment of synthetic dye-containing wastewater. |
