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Molecular typing of crude-oil-degrading bacterial strains from Riau, Indonesia |
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| รหัสดีโอไอ | |
| Creator | Irda Sayuti |
| Title | Molecular typing of crude-oil-degrading bacterial strains from Riau, Indonesia |
| Contributor | Irda Sayuti, Yusni I. Siregar, Bintal Amin, Anthoni Agustien, Adrian Hartanto |
| Publisher | Asia-Pacific Journal of Science and Technology |
| Publication Year | 2563 |
| Journal Title | Asia-Pacific Journal of Science and Technology |
| Journal Vol. | 25 |
| Journal No. | 2 |
| Page no. | 1-9 |
| Keyword | Batch fermentation, Hydrocarbonoclastic, Petroleum |
| URL Website | https://www.tci-thaijo.org/index.php/APST |
| Website title | https://so01.tci-thaijo.org/index.php/APST/article/view/240697/164317 |
| ISSN | 2539-6293 |
| Abstract | Petroleum contamination is a global concern in microbial enhanced oil recovery (MEOR) procedure, including in Riau, Indonesia, being one of the provinces with petroleum activities. These contaminants could be removed through bioremediation using crude-oil-degrading microorganisms or hence called hydrocarbonoclastic bacteria, especially the well-adapted indigenous bacterial strains from the contaminated sites. In this study, three indigenous isolates, Bacillus cereus IMB-11, Lysinibacillus fusiformis IMB-12, and Pseudomonas stutzerii IMB-15, were successfully recovered and identified from a petroleum-containing site of Chevron Pacific Indonesia (CPI) Ltd., Petapahan, Riau, Indonesia. These hydrocarbonoclastic isolates were strain-typed based on 16S rDNA and the results showed that B. cereus IMB-11 was closely related to the USA strain, L. fusiformis IMB-12 was closely related to the China and Indian strains, while P. stutzeri IMB-15 was related to strains from Africa, Asia and Europe. Also, the bioremediation assay of petroleum in a 72-h incubation experiment resulted in the removal of total petroleum hydrocarbon(s) (TPH) and chemical oxygen demand (COD) reduction by these bacterial strains during fermentation in crude oil-supplemented media (10%, v/v). Results showed that the highest TPHs removal was achieved by both B. cereus IMB-11 and P. stutzeri IMB-15 at 76.64% while L. fusiformis IMB-12 was the lowest at 62.62%. In COD analysis, the initial concentration was 15 mg/mL, also with the control flask, however, the highest COD removal was achieved by B. cereus IMB-11 at 88.55%, followed by L. fusiformis IMB-12 at 82.01%, and then P. stutzeri IMB-15 at 42.05%. Based on these results, the hydrocarbonoclastic bacterial strains have the potential to be used as bioremediation agents. |
