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Identification and Characterization of Candida tropicalis Isolated from Soil of Sugarcane Plantation in Thailand for Ethanol Production |
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| รหัสดีโอไอ | |
| Creator | Pongsanat Pongcharoen |
| Title | Identification and Characterization of Candida tropicalis Isolated from Soil of Sugarcane Plantation in Thailand for Ethanol Production |
| Contributor | Kawano-Kawada Miyuki |
| Publisher | Asia-Pacific Journal of Science and Technology |
| Publication Year | 2561 |
| Journal Title | Asia-Pacific Journal of Science and Technology |
| Journal Vol. | 23 |
| Journal No. | 3 |
| Page no. | 1-9 |
| Keyword | Candida tropicalis, thermotolerant, ethanol tolerant, ethanol production |
| URL Website | https://www.tci-thaijo.org/index.php/APST/index |
| Website title | https://tci-thaijo.org/index.php/APST/article/view/85956 |
| ISSN | 2539-6293 |
| Abstract | The thermotolerant and ethanol-producing yeasts are especially required in numerous industrial applications, such as alternative sources for bioethanol. In this study, we isolated 12 novel thermotolerant yeast strains using the enrichment technique with 4% (v/v) ethanol. The growth phenotype and ethanol fermentation activity under stress conditions were compared with the Thai industrial strain Saccharomyces cerevisiae TISTR5339. All isolated strains showed their ability to grow at 42ฐC. Among them, five isolates were found to be tolerant up to 20% (v/v) ethanol and six isolates demonstrated higher tolerance both to high temperature and ethanol up to 40ฐC with 10% (v/v) ethanol. The specific growth rates (ต) at high temperature of all strains ranged between 0.470-0.577 (h-1) at 40ฐC, which were greater than that of the reference strain. Especially one strain in this study, the strain KPC6, could convert glucose to ethanol at 14.422 g/L in 12 h and 45.232 g/L in 24 h, which showed higher productivity of ethanol than the reference strain at 42ฐC. The phylogenetic analysis based on the sequences of D1/D2 domain of 26S rDNA revealed that all strains were belonging to Candida tropicalis. Considering the results in this study, it is suggested that the Thai C. tropicalis KPC strains isolated in this study are thermo- and ethanol-tolerant ones which can be utilized as industrial microorganisms with traits that are important for future adaptation in industrial ethanol production. |
