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Oxidative Stress-Induced Apoptosis in Chronic Myelogenous Leukemia K562 Cells by an Active Compound from the Dithio- Carbamate Family |
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| รหัสดีโอไอ | |
| Creator | 1. Laya Khoshtabiat 2. Majid Mahdavi; Gholamreza Dehghan; Mohammad Reza Rashidi |
| Title | Oxidative Stress-Induced Apoptosis in Chronic Myelogenous Leukemia K562 Cells by an Active Compound from the Dithio- Carbamate Family |
| Publisher | APJCP |
| Publication Year | 2559 |
| Journal Title | Asian Pacific Journal of Cancer Prevention |
| Journal Vol. | 17 |
| Journal No. | 9 |
| Page no. | 4267-4273 |
| Keyword | Apoptosis; dithio-carbamate; oxidative stress; caspase-3; K562 cells |
| Abstract | Previous studies suggested that dithio-carbamates are potent apoptosis and anti-apoptosis inducing agents in various cancer cells. Here, the anti-proliferative and apoptosis inducing effects of a new derivative (2-NDC) from the dithio-carbamate family was examined in human leukemia K562 cells. We use thiazolyl blue tetrazolium bromide (MTT) to measure viability and cell growth inhibition. The 2-NDC showed effects on viability in a dose and time-dependent manner, inhibiting proliferation at concentrations of 10-30 _M after 24-48 hours of treatment and increasing values after 72 hours at 40-120 _M. The cytotoxic effect of the compound was calculated with an IC50 of 30 _M after 24-hour. Apoptosis induction was con rmed by acridine orange-ethidium bromide (AO/EtBr) staining, DNA fragmentation assay, ow cytometric assessment and also caspase-3 activation assay. Furthermore, enzymes level such as superoxide dismutase (SOD) and catalase (CAT) involved in oxidative stress were evaluated. The results of this study demonstrated insigni cant increase of intracellular ROS levels for 24 hours and reduction after 48-72 hours. In addition to reduction of intracellular thiol, caspase-3 like activity was also decreased in a time-dependent manner in cells treated with 2-NDC. Thus 2-NDC can be considered as a good candidate for further pharmaceutical evaluations. |
