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Epithelial-Speci c SHP1-P2 Methylation - a Novel Universal Tumor Marker for Detection of Colorectal Cancer Lymph Node Metastasis |
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| รหัสดีโอไอ | |
| Creator | 1. Prakasit Rattanatanyong 2. Somboon Keelawat; Nakarin Kitkumthorn; Apiwat Mutirangura |
| Title | Epithelial-Speci c SHP1-P2 Methylation - a Novel Universal Tumor Marker for Detection of Colorectal Cancer Lymph Node Metastasis |
| Publisher | APJCP |
| Publication Year | 2559 |
| Journal Title | Asian Pacific Journal of Cancer Prevention |
| Journal Vol. | 17 |
| Journal No. | 8 |
| Page no. | 4117-4123 |
| Keyword | SHP1-P2 ; methylation specic epithelial tissue ; lymph node metastasis ; colorectal cancer |
| Abstract | Background: Methylation of promoter 2 of the SHP1 gene is epithelial cell speci c, with reported potential as a lymph node metastatic marker. Objective: To demonstrate SHP1-P2 methylation-speci c quantitative PCR effectiveness in detecting colorectal cancer (CRC) DNA in lymph nodes. Materials and Methods: SHP1-P2 methylation levels were measured in lymph nodes of CRC patients and compared with pathological ndings and patient prognosis. Results: Lymph nodes of CRC metastatic patients without microscopically detectable cancer cells had higher SHP1-P2 methylation levels than lymph nodes of controls (p<0.001). In addition, a higher SHP1-P2 methylation level was associated with a shorter duration until adverse disease events, metastasis, recurrence and death (r2 = 0.236 and p value = 0.048). Studying two cohorts of 74 CRC patients without microscopic lymph node metastases showed that only the cohort containing samples with high SHP1-P2 methylation levels had a signi cant hazard ratio of 3.8 (95%CI = 1.02 to 14.2). Conclusions: SHP1-P2 methylation PCR can detect CRC DNA in lymph nodes even if cancer cells are not visible under a microscope, con rming applicability as a potential universal lymph node metastatic marker. |
