10.14456/apjcp.2016.205
Sex Steroids Regulate Expression of Genes Containing Long Interspersed Elements-1s in Breast Cancer Cells
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10.14456/apjcp.2016.205
Creator 1. Saichon Chaiwongwatanakul
2. Pattamawadee Yanatatsaneejit; Sissades Tongsima; Apiwat Mutirangura; Viroj Boonyaratanakornkit
Title Sex Steroids Regulate Expression of Genes Containing Long Interspersed Elements-1s in Breast Cancer Cells
Publisher APJCP
Publication Year 2559
Journal Title Asian Pacific Journal of Cancer Prevention
Journal Vol. 17
Journal No. 8
Page no. 4003-4007
Keyword Estrogen ; progesterone ; androgen ; LINE-1s ; gene experession ; breast cancer cells
Abstract Long interspersed elements-1s (LINE-1s) are dispersed all over the human genome. There is evidence that hypomethylation of LINE-1s and levels of sex steroids regulate gene expression leading to cancer development. Here, we compared mRNA levels of genes containing an intragenic LINE-1 in breast cancer cells treated with various sex steroids from Gene Expression Omnibus (GEO), with the gene expression database using chi-square analysis (http://www.ncbi.nlm.nih.gov/geo). We evaluated whether sex steroids in uence expression of genes containing an intragenic LINE-1. Three sex steroids at various concentrations, 1 and 10 nM estradiol (E2), 10 nM progesterone (PG) and 10 nM androgen (AN), were assessed. In breast cancer cells treated with 1 or 10 nM E2, a signi cant percentage of genes containing an intragenic LINE-1 were down-regulated. A highly signi cant percentage of E2-regulated genes containing an intragenic LINE-1 was down-regulated in cells treated with 1 nM E2 for 3 hours (p<3.70E-25- OR=1.91- 95% CI=2.16-1.69). Similarly, high percentages of PG or AN- regulated genes containing an intragenic LINE-1 wwere also down-regulated in cells treated with 10 nM PG or 10 nM AN for 16 hr (p=9.53E-06- OR=1.65- 95% CI=2.06-1.32 and p=3.81E-14- OR=2.01- 95% CI=2.42-1.67). Interestingly, a signi cant percentage of AN-regulated genes containing an intragenic LINE-1 was up-regulated in cells treated with 10 nM AN for 16 hr (p=4.03E-02- OR=1.40- 95% CI=1.95-1.01). These ndings suggest that intragenic LINE-1s may play roles in sex steroid mediated gene expression in breast cancer cells, which could have signi cant implications for the development and progression of sex steroid-dependent cancers.
Asian Pacific Journal of Cancer Prevention

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