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MiR-675 - hepatocellular carcinoma - proliferation - Cdc25A signaling pathway |
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| รหัสดีโอไอ | |
| Creator | 1. Ya-Qun Yu 2. Jun Weng; Shu-Qun Li; Bo Li; Jun Lv |
| Title | MiR-675 - hepatocellular carcinoma - proliferation - Cdc25A signaling pathway |
| Publisher | APJCP |
| Publication Year | 2559 |
| Journal Title | Asian Pacific Journal of Cancer Prevention |
| Journal Vol. | 17 |
| Journal No. | 8 |
| Page no. | 3881-3885 |
| Keyword | MiR-675 ; hepatocellular carcinoma ; proliferation ; Cdc25A signaling pathway |
| Abstract | Background: MicroRNAs (miRNAs) have fundamental roles in tumorigenesis. MiR-675 is upregulated in hepatocellular carcinoma(HCC) cells. However, the roles of miR-675 in hepatocellular carcinogenesis are still not fully elucidated. In this study, we focus on investigating the effect and mechanism of miR-675 in proliferation of HCC cells. Materials and Methods: The cell proliferation was measured by MTT assays after transfection with miR-675 inhibitor and miR-675 mimics in HCC cells. The expression level of miR-675 was detected by real-time quantitative reverse transcription polymerase chain reaction. Protein expression of Cdc25A was measured by western blotting analysis. Results: In MTT assays, overexpression of miR-675 promoted the proliferation of HCC cells(P<0.05. at 48 hours, P<0.01. at 72 hours) compared with the miR-675mimics control group. Downexpression of miR-675 inhibited the proliferation of HCC cells(P<0.05. at 48 hours, P<0.01. at 72 hours) compared with the miR-675inhibitor control group. In western blotting analysis, the expression level of Cdc25A was signi cantly increased (p<0.05) after treatment with miR-675 mimics. The expression level of Cdc25A was signi cantly decreased (p<0.05) after treatment with miR-675 inhibitor. Conclusions: Our results indicate that miR-675 promotes the proliferation in human hepatocellular carcinoma cells by associating with Cdc25A signaling pathway. |
