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Preparation, Characterization and Cytotoxicity of Silibinin- Containing Nanoniosomes in T47D Human Breast Carcinoma Cells |
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| รหัสดีโอไอ | |
| Creator | 1. Boshra Amiri 2. Meysam Ebrahimi-Far; Zahra Saffari; Azim Akbarzadeh; Esmaeil Soleimani; Mohsen Chiani |
| Title | Preparation, Characterization and Cytotoxicity of Silibinin- Containing Nanoniosomes in T47D Human Breast Carcinoma Cells |
| Publisher | APJCP |
| Publication Year | 2559 |
| Journal Title | Asian Pacific Journal of Cancer Prevention |
| Journal Vol. | 17 |
| Journal No. | 8 |
| Page no. | 3835-3838 |
| Keyword | Silibinin ; nanoniosomes ; breast cancer ; T-47D cells |
| Abstract | Background: Breast cancer is one of the most frequent cancer types within female populations. Silibinin is a chemotherapeutic agent ative against cancer. Niosomes are biodegradable, biocompatible, safe and effective carriers for drug delivery. Objective:To prepare nanoniosomal silibinin and evaluate its cytotoxicity inthe T-47D breast cancer cell line. Materials and Methods: Niosomes were prepared by reverse phase evaporation of a mixture of span 20, silibinin, PEG-2000 and cholesterol in chloroform and methanol solvent (1:2 v/v). The solvent phase was evaporated using a rotary evaporator and the remaining gel phase was hydrated in phosphate buffer saline. Mean size, size distribution and zeta potential of niosomes were measured with a Zetasizer instrument and then nanoparticles underwent scanning electron microscopy. The drug releasing pattern was evaluated by dialysis and the cytotoxicity of nanoniosomes in T-47D cells was assessed by MTT assay. Results: Particle size, size variation and zeta potential of the niosomal nanoparticles were measured as 178.4 ฑ 5.4 nm, 0.38 ฑ 0.09 and -15.3 ฑ 1.3 mV, respectively. The amount of encapsulated drug and the level of drug loading were determined 98.6 ฑ 2.7% and 22.3 ฑ1.8%, respectively- released drug was estimated about 18.6ฑ2.5% after 37 hours. The cytotoxic effects of nanoniosome were signi cantly increased when compared with the free drug. Conclusions: This study nding suggests that silibinin nanoniosomes could serve as a new drug formulation for breast cancer therapy. |
