Isolation and Selection of Probiotic Bacteria from Thai Traditional Fermentation Starter (Look-Pang-Khao-Mak)
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Title Isolation and Selection of Probiotic Bacteria from Thai Traditional Fermentation Starter (Look-Pang-Khao-Mak)
Creator Charoen Charoenchai
Contributor Siriwan Sangpayung, Duangjai Ochaikul
Publisher The Thai Society for Biotechnology
Publication Year 2562
Keyword Probiotic, Lactic acid bacteria, Look-Pang-Khao-Mak, Isolation
Abstract Probiotics are health-promoting microbes that provide benefits to human health by increasing the balance of microbes in the digestive system. Khao-Mak is a sweet product made from glutinous rice fermented by yeast, mold and lactic acid bacteria (LAB) occurring naturally in Look-Pang, a traditional starter culture. In the present study, lactic acid bacteria were isolated from a total of 10 Thai traditional fermentation starters (Look-Pang-Khao-Mak. One hundred twenty lactic acid bacteria isolates were screened for Gram-positive and catalase-negative characteristics. Only 68 isolates were obtained, purified and screened for their acid and bile salt tolerance. Six isolates survived at the lowest pH of 2.5 and 1% bile salt concentration. These isolates were identified as Pediococcus pentosaceus (99.73% identities) after sequencing the 16S rDNA gene. These bacteria could be considered as potential probiotic for the food industry.
Language EN
URL Website http://tsb2019.com/
Website title The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019)
Thai Society for Biotechnology

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