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The Partial Purification of Water-Soluble Cellulose from Banana Peel Extraction |
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| รหัสดีโอไอ | |
| Title | The Partial Purification of Water-Soluble Cellulose from Banana Peel Extraction |
| Creator | Jirawan Apiraksakorn |
| Contributor | Konlarat Phirom-on |
| Publisher | The Thai Society for Biotechnology |
| Publication Year | 2562 |
| Keyword | Cellulose, Enzymatic Hydrolysis, Ethanol Precipitatio |
| Abstract | Banana peel is one of the lignocellulosic wastes from food processing and often used as a raw material for cellulose extraction. To obtain watersoluble cellulose, the extracted cellulose was hydrolysed by cellulase. The purification was needed since the process of hydrolysis provided mixed products of glucose, cello-oligosaccharides, as well as soluble cellulose. Thus, the aim of this study was to partially purify the soluble cellulose from the enzymatic hydrolysate. Initially, cellulose fiber was extracted from banana peel by alkaline and bleaching treatment. Cellulose extracted was then hydrolyzed by commercial cellulase. The hydrolysate was purified by using ethanol precipitation. The purification was investigated by thin layer chromatography and glucose kit assay. The results revealed that the ethanol could precipitate the hydrolysate into 2 phases, the supernatant and the ethanol insoluble pellet. The thin layer chromatography depicted that glucose, cellobiose, cellotriose, and cellotetraose were detected in the supernatant. The ethanol insoluble pellet was water-soluble cellulose. Moreover, the glucose kit assay could not detect any glucose in the pellet. Therefore, this convenient purification could be effectively used for water-soluble cellulose production. This soluble fiber would be further studied as a food additive. |
| Language | EN |
| URL Website | http://tsb2019.com/ |
| Website title | The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019) |
