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Metformin Suppresses Epithelial Cell Adhesion Through ROS Production Involving Apoptosis in Cervical Cancer Cells |
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| รหัสดีโอไอ | |
| Title | Metformin Suppresses Epithelial Cell Adhesion Through ROS Production Involving Apoptosis in Cervical Cancer Cells |
| Creator | Pilaiwanwadee Hutamekalin |
| Contributor | Wanumaidah Saengmolee, Wanida Sukketsiri |
| Publisher | The Thai Society for Biotechnology |
| Publication Year | 2562 |
| Keyword | Metformin, ROS, Cell Adhesion, E-Cadherin, Apoptosis |
| Abstract | Cervical cancer is one of the most prevalent cancer among female after breast cancer and its mortality rate is the highest in women malignant tumor of reproductive system. Epidemiological study indicates that metformin, an anti-diabetes drug, has an anti-proliferation effect with apoptotic-response mitochondrial signaling cascade in various cancers. However, the effect of metformin-mediated apoptosis involves in epithelial cell adhesion is not well characterized. Therefore, the mechanism underlying metformin-induced apoptosis related to epithelial cell adhesion expression in HeLa cells was investigated. The anti-proliferation effect of metformin in the HeLa cells was determined by MTT assay. Cell apoptosis was detected using Flow cytometry assay. The levels of E-cadherin (epithelial cell adhesion marker) expression were performed using Western blot analysis as well as the intracellular ROS levels were measured by DCFH-DA fluorimetric assay. The results revealed that metformin inhibited cell proliferation and induced cell apoptosis. Moreover, metformin suppressed the expression of E-cadherin through the increased ROS generation that was completely rescued by Nacetyl cysteine (NAC), ROS scavenger. Taken together, our results suggested that metformin-mediated apoptosis involved in the elevated ROS suppressing the expression of epithelial cell adhesion. |
| Language | EN |
| URL Website | http://tsb2019.com/ |
| Website title | The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019) |
