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Inhibition of Hydrogen Synthesis Increased Accumulations of Pigments in Cyanobacterium Synechocystis sp. PCC 6803 |
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| รหัสดีโอไอ | |
| Title | Inhibition of Hydrogen Synthesis Increased Accumulations of Pigments in Cyanobacterium Synechocystis sp. PCC 6803 |
| Creator | Tanakarn Monshupanee |
| Contributor | Nannaphat Sukkasam |
| Publisher | The Thai Society for Biotechnology |
| Publication Year | 2562 |
| Keyword | Synechocystis sp. PCC 6803, Cyanobacteria, Hydrogenase, Allophycocyanin, Phycocyanin |
| Abstract | Cyanobacterium Synechocystis sp. PCC 6803 produces one hydrogen gas molecule from two NADPH molecules via the only catalysis of the enzyme [NiFe]-hydrogenase composing of several subunits: HoxE, HoxF, HoxU, HoxY, and HoxH. Effects of the inhibition of hydrogen synthesis on the accumulation levels of other bioproducts have yet to be determined. Here, the two main catalytic subunits of [NiFe]-hydrogenase (HoxY and HoxH) were inactivated by the deletion of both hoxY and hoxH gene. Inactivation of both hoxY and hoxH gene did not have a deleterious effect to cell growth, suggesting that the hydrogen synthesis is not an essential process for cell viability. In addition, inactivation of both hoxY and hoxH genes significantly increased the accumulation levels of phycocyanin by 1.55 fold, and allophycocyanin by 1.49 fold. The biosynthesis of hydrogen (via hydrogenase), phycocyanin and allophycocyanin require the same substrate - NADPH. Thus, the inhibition of the hydrogen synthesis (via hoxY and hoxH inactivation) might help increase cellular NADPH level and mediate the enhanced production of phycocyanin and allophycocyanin. |
| Language | EN |
| URL Website | http://tsb2019.com/ |
| Website title | The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019) |
