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Immobilization of Alternansucrase on Chitosan Beads |
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| รหัสดีโอไอ | |
| Title | Immobilization of Alternansucrase on Chitosan Beads |
| Creator | Manchumas Prousoontorn |
| Contributor | Kusuma Thongplaew, Thanapon Charoenwongpaiboon, Karan Wangpaiboon, Rath Pichyangkura |
| Publisher | The Thai Society for Biotechnology |
| Publication Year | 2562 |
| Keyword | Immobilization, Alternansucrase, Chitosan Beads, Alternan |
| Abstract | Alternansucrase (Alt, EC 2.4.1.140) is an enzyme that catalyzes transferring of an ?-D-glucosyl residue from sucrose to another sucrose molecule which acts as an acceptor, thereby creating a glucan with alternating ?-1,6 and ?-1,3-glycosidic linkages, known as alternan. The truncated alt (?Calt) gene was expressed in E. coli BL21(DE3) and recombinant Alt was partially purified by DEAE anion exchange chromatography. The recombinant Alt possessed an optimum temperature and pH of 40?C and 4.0, respectively. In order to reuse the enzyme and to enhance its stability, Alt was immobilized on a chitosan support by covalent method using glutaraldehyde as a crosslinking agent. Chitosan beads were activated under optimal immobilization condition which was 0.5% (w/v) glutaraldehyde in 50 mM citrate buffer pH 6.0 at 4?C overnight. After that, the activated chitosan beads were incubated with an enzyme solution (30 U or 0.69 mg/g of beads) at 4?C overnight. The immobilized enzyme will be further employed for the production of alternan. |
| Language | EN |
| URL Website | http://tsb2019.com/ |
| Website title | The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019) |
