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Design, Generation and Protein Expression of the EpitopeBased PEDV (Porcine Epidemic Diarrhea Virus) Vaccine |
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| รหัสดีโอไอ | |
| Title | Design, Generation and Protein Expression of the EpitopeBased PEDV (Porcine Epidemic Diarrhea Virus) Vaccine |
| Creator | Yaowaluck Maprang Roshorm |
| Contributor | Kanokporn Polyiam, Marasri Ruengjitchatchawalya, Teerapan Laomettachit, Kanokwan Poomputsa, Phenjun Mekvichitsaeng |
| Publisher | The Thai Society for Biotechnology |
| Publication Year | 2562 |
| Keyword | PEDV, Epitope-Based Vaccine, DNA Vaccine, Adenovirus, E . coli |
| Abstract | 1. Porcine Epidemic Diarrhea (PED) continues to cause huge economic losses in swine industry worldwide. Porcine Epidemic Diarrhea Virus (PEDV), the causative agent of PED, exhibits highly genetic variation 2. thus, a universal vaccine is desirable. Our main goal is to develop a universal vaccine using multi-epitope strategy. In this study, we designed, generated and studied the expression of the PEDV multi-epitope vaccines. Two new vaccine antigens, epiS(MHC) and epiS(CTL), were designed to contain CD8 T cell epitopes obtained from in silico prediction of the PEDV spike protein (S). The vaccines were then developed using 3 different vaccine systems. In HEK293A, the antigens produced by DNA vaccines and adenovirus-based vaccines exhibited only small amount of the protein. When expressed in E . coli expression system, only the epiS(MHC) protein displayed full-length and degraded forms of the protein when it is detected by Western blot. The results of protein expression by 3 different vaccine platforms suggest that epitope-based vaccine can be very unstable and susceptible to degradation and further modification is important to help improve stability and functionality of the vaccines. |
| Language | EN |
| URL Website | http://tsb2019.com/ |
| Website title | The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019) |
