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Comparison of pilot- and laboratory-scale centrifuge recovery and dewatering performances on beta-glucan production |
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| รหัสดีโอไอ | |
| Title | Comparison of pilot- and laboratory-scale centrifuge recovery and dewatering performances on beta-glucan production |
| Creator | Panwajee Payongsri |
| Contributor | Sasimaporn Wongchana, Nitchanan Wilailoet, Punchanit Puangnate, Kamolchai Tongpueak, Manop Suphantharika |
| Publisher | The Thai Society for Biotechnology |
| Publication Year | 2562 |
| Keyword | Beta-Glucan, Scale-Up, Centrifugation |
| Abstract | Beta-glucan production involves separation of yeast cell wall from soluble cellular components and centrifugation is commonly employed. Poor liquid-solid separation and solid recovery, however, are common obstacles during process scale- up. In this study, the recovery and dewatering performance of floor-type centrifuge (Beckman J-6M/E) and pilot scale discstack centrifuge (Westfalia separator SA1-01-175 with Double Centripetal Pump) were compared. Centrifuges at both scales were used to separate cell wall from yeast autolysate as well as to wash cell wall. Centrifugation of yeast autolysate and homogenate (500 mL) in a 1-L bucket at 3500 rpm for 40 min with 15% w/w, the floor-type centrifuge could achieve 30% w/w sediment. The disc-stack centrifuge for pilot scale centrifugation, on the other hand, could achieve up to 20% w/w sediment when feeding samples with 1-5% w/w at 1 L/min, 9,000 rpm. Wetter sediment at a pilot-scale did not lead to higher contaminants in the finished product, allowing the process scale up. Before spray drying, the beta-glucan recovery yield in laboratory- and pilot-scale were 21% and 10%, respectively. The major loss during pilot-scale production was, indeed, due to disc-stack centrifuge. It is important that the flow rate used has to be compromised between the processing time required and % solid recovery. Flow duration, however, did not influence the recovery until the point that the bowl was completely full. |
| Language | EN |
| URL Website | http://tsb2019.com/ |
| Website title | The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019) |
