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Identification of Cellulase-Producing Bacteria from Soil in Nasinuan Forest, Kantarawichai District, Mahasarakham Province |
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| รหัสดีโอไอ | |
| Title | Identification of Cellulase-Producing Bacteria from Soil in Nasinuan Forest, Kantarawichai District, Mahasarakham Province |
| Creator | Manatchanok Yotchaisarn |
| Contributor | Worachot Saengha, Thipphiya Karirat, Sirirat Deeseenthum, Kedsukon Maneewan, Vijitra Luang-In |
| Publisher | Mahasarakham University |
| Publication Year | 2561 |
| Keyword | Carboxymethyl Cellulose, Cellulase, Bacteria, Soil, Industry |
| Abstract | This study aimed to isolate and identify bacteria that can produce cellulase enzyme from Nasinuan forest, Kantarawichai District, Mahasarakham Province, Thailand. Five bacterial isolate representatives out of 82 isolates with cellulase-producing capacity on 1% carboxymethyl cellulose (CMC) agar were chosen for strain identification. Five bacteria were gram-positive, rod shaped with similar colony morphologies and identified as Bacillus spp. using 16S rRNA gene analysis. The highest halo:colony ratios on CMC agar were in the order: Bacillus subtilis 1.1CL4 > B. licheniformis 1.1CL3 = B. licheniformis 1.2CL3 > B. licheniformis 1.1CL1 > B. subtilis 1.2CL2. Their closest relatives were Bacillus spp. found in Sudan, Saudi Arabia, India and Pakistan. These cellulaseproducing bacteria can be applied for use in a wide variety of industries such as dessert production, beverage production, textile production and dairy industry. |
| Language | English |
