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ลำดับนิวคลีโอไทด์บางส่วนของยีน CHD-W และ CHD-Z เพื่อการระบุเพศของนกปรอดหัวโขน (Partial nucleotide sequences of CHD-W and CHD-Z genes for sex identification of Red-whiskered Bulbul (Pycnonotus jocosus)) |
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| รหัสดีโอไอ | |
| Creator | 1. Somchart Tana 2. Dutrudi Panprommin |
| Title | ลำดับนิวคลีโอไทด์บางส่วนของยีน CHD-W และ CHD-Z เพื่อการระบุเพศของนกปรอดหัวโขน (Partial nucleotide sequences of CHD-W and CHD-Z genes for sex identification of Red-whiskered Bulbul (Pycnonotus jocosus)) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2557 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 7 |
| Journal No. | 2 |
| Page no. | in press |
| Keyword | CHD-W and CHD-Z genes, sex Identification, Red-whiskered Bulbul (Pycnonotus jocosus), polymerase chain reaction |
| ISSN | 8578664 |
| Abstract | This study was aimed to determine the partial nucleotide sequences of chromo-helicase-DNA-binding protein (CHD) genes for sex identification of red-whiskered bulbul (Pycnonotus jocosus) using polymerase chain reaction technique. The partial sequences of CHD-W and CHD-Z genes were amplified with the P8/P2 primers from root hairs of known-sex adults and unknown-sex chicks. This process was carried out without the DNA extraction or DNA purification, thus saving time. The twenty known-sex adults were determined; two DNA fragments were amplified in females and one in males. In females, the 384-bp fragment of CHD-W and 332-bp fragment of CHD-Z genes were amplified. While only 332-bp fragment of CHD-Z gene was amplified in males. The sex identification using PCR method had 100% accuracy. The sexes of unknown-sex chicks were also identified and produced the same fragments. The results of this study can be used to plan the breeding and conservation of this species. In addition, the CHD-W and CHD-Z sequences of red-whiskered bulbul was the highest similarity to the light-vented bulbul (Pycnonotus sinensis), belonging to the same genus Pycnonotus. The phylogenetic tree of four bird species including red-whiskered bulbul (Pycnonotus jocosus) light-vented bulbul (Pycnonotus sinensis) skylark (Alauda arvensis) and common swift (Apus apus) was constructed for evolutionary analysis. The nodes of tree were separated clearly followed the bird taxonomic system. |
