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Plasma Technology and Abiotic Elicitor Effectively Increased Isothiocyanates, Bioactive Compounds and Cytotoxicity against Caco2 Cells in Mustard Green Microgreen Extract |
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| รหัสดีโอไอ | |
| Title | Plasma Technology and Abiotic Elicitor Effectively Increased Isothiocyanates, Bioactive Compounds and Cytotoxicity against Caco2 Cells in Mustard Green Microgreen Extract |
| Creator | Worachot Saengha |
| Contributor | Thipphiya Karirat, Benjaporn Buranrat, Khanit Matra, Sirirat Deeseenthum, Vijitra Luang-In |
| Publisher | Faculty of Technology, Mahasarakham University |
| Publication Year | 2562 |
| Keyword | Isothiocyanate, Cytotoxicity, Mustard green, Plasma, Elicitor |
| Abstract | The objective of this study was to study the effect of plasma technology and abiotic elicitor on percent germination, length of stem, fresh weight, total isothiocyanate, bioactive compounds and cytotoxicity against Caco2 cells of mustard green (Brassica juncea L. Czern. et Coss) microgreens germinated on vermiculite at 25 ?C in 12 h light/12 dark cycle for 7 days. The results showed that plasma treatment and abiotic elicitor including sucrose, NaCl and CaCl2 did not have any effect on the % germination, but it affected the length of mustard green stems. Using only plasma treatment was able to increase the total isothiocyanate content (2.65 mmol/100g DW), total phenolic content (5.08 mg GAE/g DW), total flavonoid content (0.17 mg RE/g DW), antioxidant activity by DPPH assay (7.93 mg Trolox /g DW) and FRAP assay (15.42 mg Fe2+/g DW). The lowest IC50 of mustard green extract against CaCo2 cells was found when incubated for 72 h at 30.61 ?g/mL. Treated Caco2 cells showed signs of shrinkage and membrane blebbing i.e. apoptosis. This study indicated that using plasma technology can improve the physiology of mustard microgreens and enhance the isothiocyanate content, bioactive compounds and cytotoxicity. Therefore, this technology can be used for improving the nutritive value and phytochemicals of microgreens as functional foods. |
| Language | EN |
| URL Website | https://techno2.msu.ac.th/icofab2019/abstract-collection-and-proceedings/ |
| Website title | The 6th International Conference on Food Agriculture & Biotechnology(ICoFAB 2019) |
