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Random Mutagenesis of Aspergillus sclerotiorum PSU-RSPG 178 for Improvement a Lovastatin Production |
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| รหัสดีโอไอ | |
| Title | Random Mutagenesis of Aspergillus sclerotiorum PSU-RSPG 178 for Improvement a Lovastatin Production |
| Creator | Monwadee Wonglapsuwan |
| Contributor | Supawan Meena, Wilaiwan Chotigea, Yaowapa Sukpondma, Souwalak Phongpaichit, Vatcharin Rukachaisirikul |
| Publisher | Faculty of Technology, Mahasarakham University |
| Publication Year | 2562 |
| Keyword | Lovastatin, Aspergillus, Aspergillus sclerotiorum PSU-RSPG 178, random mutagenesis |
| Abstract | Aspergillus sclerotiorum PSU-RSPG 178 is a natural fungus producing lovastatin which is commonly used as cholesterol reduction medication. Random mutagenesis method was used to increase the production of lovastatin via UV radiation (255 nm) with a different time of exposure at 30, 45 and 60 min, fifty colonies appeared and screened for lovastatin production by agar plug and Neurospora crassa bioassay. The results showed seven colonies had greater diameter of clear zone than that of wild type. After analysis of the quantity of lovastatin by HPLC, the mutant colonies from UV mutation at 30 min provided the maximum amount of lovastatin at 354.69 mg/L compared with that from the control at 113.4 mg/L. However, after sub-culture until 3rd generation, the potential in lovastatin production declined. This evidence indicated that UV mutagenesis could be used for improving the lovastatin production |
| Language | EN |
| URL Website | https://techno2.msu.ac.th/icofab2019/abstract-collection-and-proceedings/ |
| Website title | The 6th International Conference on Food Agriculture & Biotechnology(ICoFAB 2019) |
