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Screening and Identification of Bacteria that Produce Chitinase Enzymes from Soil in Na Si Nuan Forest, Maha Sarakham, Thailand |
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| รหัสดีโอไอ | |
| Title | Screening and Identification of Bacteria that Produce Chitinase Enzymes from Soil in Na Si Nuan Forest, Maha Sarakham, Thailand |
| Creator | Ketsara Suwunnapukdee |
| Contributor | Sirirat Deeseenthum, Vijitra Luang-In, Kedsukon Maneewan |
| Publisher | Faculty of Technology, Mahasarakham University |
| Publication Year | 2562 |
| Keyword | Chitinase enzymes, Bacteria, Soil, Na Si Nuan Forest, Agriculture. |
| Abstract | This research aimed to screen the bacteria that produce chitinase enzymes from soil in Na Si Nuan forest located at Kantarawichai District, Maha Sarakham Province, Thailand. The conservation project in response to Plant Genetic Conservation Project under the Royal initiative of Her Royal Highness Princess Maha Chakri Sirindhorn has occupied the community forest of 19.2 ha in this study. Isolation of bacteria was performed from soil on chitin agar. Our results were found two isolates of chitinase-positive bacterial strains. The isolate of 3.2 CT1 was showed the highest of halo: colony ratios with 4.5. The morphology isolate of 3.2 CT1 is gram negative, and rod shaped. The optimum temperature for chitinase activity was 40 ?C with enzyme activity of 0.40 ? 0.01 U/mL and given a specific enzyme activity of 0.49 ? 0.01 U/mg. protein. The optimum pH was 6.0 with activity of 0.70 ? 0.01 U /mL. This chitinase may be used for agricultural purposes, such as plant pathogen inhibition and as insecticide for insect pest control. |
| Language | EN |
| URL Website | https://techno2.msu.ac.th/icofab2019/abstract-collection-and-proceedings/ |
| Website title | The 6th International Conference on Food Agriculture & Biotechnology(ICoFAB 2019) |
