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Biofilm Formation, Proteinase and Phospholipase Activities of Candida parapsilosis Isolated from Environment |
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| รหัสดีโอไอ | |
| Creator | Srisuda Pannanusorn |
| Title | Biofilm Formation, Proteinase and Phospholipase Activities of Candida parapsilosis Isolated from Environment |
| Contributor | Natnapat Nakkao, Nattida Cholnakasem |
| Publisher | Thammasat University |
| Publication Year | 2563 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 9 |
| Journal No. | 1 |
| Page no. | 129-141 |
| Keyword | Candida parapsilosis, biofilm formation, proteinase, phospholipase |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | Candida parapsilosis, although, is a human commensal of epithelial and mucosal tissues. This species is also frequently isolated from environments. C. parapsilosis is notable for the ability to form biofilms on medical devices. Therefore, it is frequently associated with nosocomial outbreaks. In addition, ability of C. parapsilosis producing secrete proteinase and phospholipase can causes more severe infection. The present study aimed to determine biofilm formation using crystal violet staining and hydrolytic enzyme production using spot assay on relevant agar medium of C. parapsilosis isolated from environment. In total, 8 of 30 yeast isolates (26.66 percent) obtained from environment were C. parapsilosis. Biofilm formation of the isolated C. parapsilosis was variable. All of the isolates revealed the higher biofilm formation at room temperature (30 degree Celcius) compared to 37 degree Celcius. All the isolates showed moderate to high activity of proteinase but no activity of phospholipase. In contrast to biofilm formation, proteinase production of the isolates was increased at 37 degree Celcius. In conclusion, temperature is one important factor affecting biofilm formation and proteinase production. The formation of the biofilm is possibly beneficial for the isolates to live in the hostile environment. However, production of proteinase may play important role for C. parapsilosis to cause infection in human. |
