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Evaluation of Disease Resistance of the Cassava Lines Introduced from Nigeria to Sri Lankan Cassava Mosaic Virus, the Causal Agent of Cassava Mosaic Disease, under Greenhouse Conditions |
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| รหัสดีโอไอ | |
| Creator | Chonticha Rakkrai |
| Title | Evaluation of Disease Resistance of the Cassava Lines Introduced from Nigeria to Sri Lankan Cassava Mosaic Virus, the Causal Agent of Cassava Mosaic Disease, under Greenhouse Conditions |
| Contributor | Tdawan Chomdate, Wanich Khampanich, Phannipa Paechaisri, Danai Chaireunkaew, Phoowanarth ManeechoatTdawan Chomdate, Wanich Khampanich, Phannipa Paechaisri, Danai Chaireunkaew, Phoowanarth Maneechoat |
| Publisher | Department of Agriculture |
| Publication Year | 2565 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 40 |
| Journal No. | 3 |
| Page no. | 226-237 |
| Keyword | Cassava mosaic virus, disease resistance, grafting inoculation, disease severity, virus detection |
| URL Website | https://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | The first outbreak of cassava mosaic disease (CMD) in Thailand was reported in 2018. The causal virus was Sri Lankan cassava mosaic virus (SLCMV). Due to the uses of the infected cassava cuttings for planting the disease widely spread across the country and caused severe tuber yield losses, . One approach applied to control CMD was the use of CMD-resistant cassava lines. In this research, five cassava lines resistant to CMD were introduced from Nigeria and evaluated for resistance to SLCMV under greenhouse conditions. Artificial inoculation was performed by top grafting the virus-free Nigerian cassava lines onto the SLCMV-infected shoots of CMR43-08-89, a susceptible cassava line. Disease severity and incidence were recorded 2-8 weeks after inoculation (WAI). Results revealed that all five Nigeria cassava lines, i,e. IITA-TMS-IBA 980581, IITA-TMS-IBA 972205, IITA-TMS-IBA 980505, IITA-TMS-IBA 920057 and TME B419 were susceptible to SLCMV with 100% disease incidence. All grafted plants gave positive SLCMV detection by PCR by showing a 747 bp fragment of the viral replicase gene. The ranges of mean severity score of the Nigerian cassava lines were 2.11-2.56. The mean severity score of the C33 control line was 1.89 and categorized as resistant (R), while the score of TME3 control line was 2.78 and categorized as susceptible (S). The detection of SLCMV in both symptomatic and asymptomatic tested plants indicated the tolerance reaction of some cassava lines which contained virus but showed less symptom. |
