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EXTRACTION OF ANTIOXIDANT FROM COCOA BEAN SHELL |
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| รหัสดีโอไอ | |
| Creator | Sarita Sangthong |
| Title | EXTRACTION OF ANTIOXIDANT FROM COCOA BEAN SHELL |
| Contributor | Sareerut Muensri, Attaphon Juiseekaew, Benjarat Suwannawong |
| Publisher | Pibulsongkram Rajabhat University |
| Publication Year | 2567 |
| Journal Title | Life Sciences and Environment Journal |
| Journal Vol. | 25 |
| Journal No. | 2 |
| Page no. | 507-518 |
| Keyword | cocoa bean shell, antioxidant, polyphenols, theobromine |
| URL Website | https://ph01.tci-thaijo.org/index.php/psru/index |
| Website title | Life Sciences and Environment Journal |
| ISSN | 2773-9201 |
| Abstract | Cocoa bean shell (CBS) is a by-product from the cocoa industry that contains various phytochemicals that could be applied as functional ingredient development. This study investigated the use of various polarity solvents namely, aqueous, ethanol 95% (95% EtOH) and 50% (50% EtOH), and propylene glycol 100% (100% PG), and 50% (50% PG). CBS extracted by 50% EtOH and 50% PG showed significantly the highest total phenolic content (TPC), total flavonoid content (TFC), free radical scavenging activity (DPPH), and ferric reducing power (FRAP) but not significantly different from each other. While HPLC analysis showed that 50% EtOH extract possessed the highest caffeine, catechin, and theobromine resulting in 8.360, 0.586, and 47.606 mg/kg, respectively in 50% EtOH, followed by 50% PG which were 7.992, 0.395, and 38.463 mg/kg, respectively. The stability testing at 25°C, 4°C, and 37°C for 28 days found that the antioxidant activity of 50% EtOH and 50% PG extracts was highly stable. A strong correlation of antioxidant activities with TPC and TFC was found. This study can conclude that cocoa bean shell extracted with either 50% EtOH or 50% PG are suitable choices for further studies. |
