EXTRACTION CONDITIONS ON A CRUDE SULFATED POLYSACCHARIDE EXTRACT CONTENT, PHENOLIC COMPOUNDS, AND ANTIOXIDANT ACTIVITIES OF CAULERPA LENTILLIFERA
รหัสดีโอไอ
Creator Numphon Thaiwong
Title EXTRACTION CONDITIONS ON A CRUDE SULFATED POLYSACCHARIDE EXTRACT CONTENT, PHENOLIC COMPOUNDS, AND ANTIOXIDANT ACTIVITIES OF CAULERPA LENTILLIFERA
Contributor Natta Kachenpukdee, Tanayt Sinprachim, Supraewpan Lohalaksanadech, Thongchai Nitiratsuwan, Hathaitip Tongdoung
Publisher Pibulsongkram Rajabhat University
Publication Year 2567
Journal Title Life Sciences and Environment Journal
Journal Vol. 25
Journal No. 2
Page no. 481-493
Keyword Caulerpa lentillifera, crude sulfated polysaccharide extract, standard antioxidant agents
URL Website https://ph01.tci-thaijo.org/index.php/psru/index
Website title Life Sciences and Environment Journal
ISSN 2773-9201
Abstract Caulerpa lentillifera is a type of seaweed that benefits the consumer's health, so its price is quite high. Then, C. lentillifera is encouraged to be commercially cultivated. This study focused on the extraction of crude sulfated polysaccharide extract from C. lentillifera (SFPS), which is a bioactive compound. The study examined the extraction conditions of SFPS using different ratios of C. lentillifera to water (1:5, 1:10, and 1:15) and different extraction times (30, 60, and 90 minutes). The results showed that a ratio of 1:10 and an extraction time of 60 minutes were optimal conditions for obtaining the highest values of yield, phenolic compounds, and antioxidant activity (using the ABTS and DPPH methods). These values were about 0.85±0.10%, 31.99±0.46 mg GAE/g extract, 44.19±1.51% inhibition of the ABTS method, and 30.55±1.30% inhibition of the DPPH method, respectively (p≤0.05). The proximate analysis of SFPS revealed moisture, protein, fat, and ash contents of approximately 2.37±0.18, 1.49±0.18, 0.17±0.03 and 12.23±0.06%, respectively. The SFPS had a phenolic compound content of 31.99±0.46 mg GAE/g extract, and exhibited antioxidation activities of 44.19±1.51% inhibition by the ABTS method and 30.55±1.30% inhibition by the DPPH method (p≤0.05). Comparing the antioxidation activities among SFPS and standard antioxidant agents such as gallic acid and butylated hydroxytoluene (BHT), the % inhibition by ABTS and DPPH methods could be ordered from highest to lowest as follows: BHT, gallic acid, and SFPS, respectively.
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