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EXTRACTION CONDITIONS ON A CRUDE SULFATED POLYSACCHARIDE EXTRACT CONTENT, PHENOLIC COMPOUNDS, AND ANTIOXIDANT ACTIVITIES OF CAULERPA LENTILLIFERA |
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| รหัสดีโอไอ | |
| Creator | Numphon Thaiwong |
| Title | EXTRACTION CONDITIONS ON A CRUDE SULFATED POLYSACCHARIDE EXTRACT CONTENT, PHENOLIC COMPOUNDS, AND ANTIOXIDANT ACTIVITIES OF CAULERPA LENTILLIFERA |
| Contributor | Natta Kachenpukdee, Tanayt Sinprachim, Supraewpan Lohalaksanadech, Thongchai Nitiratsuwan, Hathaitip Tongdoung |
| Publisher | Pibulsongkram Rajabhat University |
| Publication Year | 2567 |
| Journal Title | Life Sciences and Environment Journal |
| Journal Vol. | 25 |
| Journal No. | 2 |
| Page no. | 481-493 |
| Keyword | Caulerpa lentillifera, crude sulfated polysaccharide extract, standard antioxidant agents |
| URL Website | https://ph01.tci-thaijo.org/index.php/psru/index |
| Website title | Life Sciences and Environment Journal |
| ISSN | 2773-9201 |
| Abstract | Caulerpa lentillifera is a type of seaweed that benefits the consumer's health, so its price is quite high. Then, C. lentillifera is encouraged to be commercially cultivated. This study focused on the extraction of crude sulfated polysaccharide extract from C. lentillifera (SFPS), which is a bioactive compound. The study examined the extraction conditions of SFPS using different ratios of C. lentillifera to water (1:5, 1:10, and 1:15) and different extraction times (30, 60, and 90 minutes). The results showed that a ratio of 1:10 and an extraction time of 60 minutes were optimal conditions for obtaining the highest values of yield, phenolic compounds, and antioxidant activity (using the ABTS and DPPH methods). These values were about 0.85±0.10%, 31.99±0.46 mg GAE/g extract, 44.19±1.51% inhibition of the ABTS method, and 30.55±1.30% inhibition of the DPPH method, respectively (p≤0.05). The proximate analysis of SFPS revealed moisture, protein, fat, and ash contents of approximately 2.37±0.18, 1.49±0.18, 0.17±0.03 and 12.23±0.06%, respectively. The SFPS had a phenolic compound content of 31.99±0.46 mg GAE/g extract, and exhibited antioxidation activities of 44.19±1.51% inhibition by the ABTS method and 30.55±1.30% inhibition by the DPPH method (p≤0.05). Comparing the antioxidation activities among SFPS and standard antioxidant agents such as gallic acid and butylated hydroxytoluene (BHT), the % inhibition by ABTS and DPPH methods could be ordered from highest to lowest as follows: BHT, gallic acid, and SFPS, respectively. |
