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Active compounds, free radicals scavenging and tumor-necrosis factor (TNF-?) inhibitory activities of star fruit-sweet type (Averrhoa carambola L.) in vitro |
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| รหัสดีโอไอ | |
| Creator | 1. Yothin Pothasuk 2. Supawatchara Singhatong 3. Surapol Natakankitkul 4. Nathupakorn Dechsupa 5. Penpitcha Wanachantararak 6. Chanchai Dechthummarong 7. Jirakrit Leelarungrayub |
| Title | Active compounds, free radicals scavenging and tumor-necrosis factor (TNF-?) inhibitory activities of star fruit-sweet type (Averrhoa carambola L.) in vitro |
| Publisher | Faculty of Associated Medical Sciences, Chiang Mai University |
| Publication Year | 2563 |
| Journal Title | Journal of Associated Medical Sciences |
| Journal Vol. | 53 |
| Journal No. | 1 |
| Page no. | 19-28 |
| Keyword | Active compounds, Free radical scavenging, Anti-inflammation, Star fruit |
| URL Website | https://www.tci-thaijo.org/index.php/bulletinAMS/index |
| Website title | Journal of Associated Medical Sciences |
| ISSN | 25396056 |
| Abstract | Background: Star fruit (Averrhoa carambola L.) is a seasonal fruit that originated in Southeast Asia, including Thailand. There are two distinct types; sour and sweet, with various active compounds such as saponins, flavonoid C-glycosides, tannin, L-ascorbic acid, (-) epicatechin and gallic acid. However, the active compounds, especially total phenolic compound of star fruit-sweet type, have not been fully investigated. Objectives: Thus, this study aimed to investigate the active compounds, radical scavenging and anti-inflammatory activity of star fruit-sweet type in vitro. Materials and methods: Total phenolic compound, total flavonoids and L-ascorbic acid in extract were evaluated by Folin-Ciocalteau reagent, aluminum chloride colorimetric assay and high-performance liquid chromatography, respectively. Radical scavenging activity on 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) cation radicles and nitric oxide (NO), and protective activity of glutathione (GSH) from free radicals generated by high voltage-stimulation in plasma micro/nano bubble water were studied. Finally, the anti-inflammatory activity on tumor necrosis factor-alpha (TNF-?) release was performed in lipopolysaccharide (LPS)-activated macrophage cells. Results: One hundred grams star fruit extract showed total phenolic compounds as 5.12ฑ0.24 ตg of gallic acid, total flavonoids as 0.185ฑ0.008 ตg of quercetin and 5.24ฑ0.55 ตg of ascorbic acid. One gram of Star fruit extract, equal to 722.71ฑ12.25 ตg of gallic acid (GAE), showed radical scavenging activity on ABTS?+. Star fruit extract (27.48ฑ1.8 ตg of IC50), standard gallic (24.28ฑ2.6 ตg of IC50) and ascorbic acid (54.98ฑ2.5 ตg of IC50) also showed radical scavenging activity on NO, respectively. Whereas Star fruit extract also scavenged free radicals generated by 10 min of high voltage (HV) stimulation in micro/nano-bubble water, and protected GSH with dose response (125-1,000 ตg/mL), in the same way as standard ascorbic acid. Finally, the star fruit extract, and also standard hydrocortisone, had anti-TNF-? releases with dose responses. Conclusion: The star fruit extract-sweet type has antioxidant activity with phenolic compound, flavonoids, and ascorbic acid, as well as having anti-inflammatory activity. |
