รหัสดีโอไอ 10.14455/TSB.res.2019.57
Title Upscaling of RS87 endospore production process - from flask to semi-industrial scale cultivation
Creator Akarawit Jenjitwanich
Contributor 1. Kanchalee Jetiyanon
2. Thunyarat Pongtharangkul
Publisher The Thai Society for Biotechnology
Publication Year 2019
Keyword Upscaling Cultivation ,PGPR ,B . cereus RS87 ,Endospore
Abstract Utilization of PGPR (plant growth promoting rhizobacteria) has been documented to enhance crop yield and reduce the usage of agrochemicals. Bacillus cereus strain RS87 has been proven for its PGPR properties as well as an ability for 50% chemical fertilizer substitution in several crops (i.e. rice, pepper, cucumber and tomato). Nonetheless, the conventional process for RS87 endospore production using 0.5xTSB (tryptic soy broth) in a flask-scale cultivation was not a cost-effectiveness and resulted in a low spore yield (2x107 spores/mL after 4 days). In order to make the process compatible for a large-scale production, an effective low-cost cultivation medium is inevitable. Beside, the cultivation conditions in a bioreactor-scale must be established. An optimized cultivation medium previously developed for RS87 by Jenjitwanich et al. (2018) or omCCY was proven to yield good growth and endospore production at a flask-scale. In this study, omCCY was employed in an upscaling cultivation using a 5 L-bioreactor. Effects of %dissolved oxygen (%DO) and mode of cultivation (batch vs fed-batch) on growth and endospore production of RS87 were investigated. The 24 h-batch cultivation with low %DO (10-20%) yielded a comparable maximum spore count (3.4x108 spores/mL after 24 h) to that of flask-scale cultivation. Maintaining of %DO at a moderate level (40-50%) allowed us to obtain the maximum spore count of 2.5x108 spores/mL within only 12 h. Finally, the fed-batch cultivation with pulse additions of nutrients (glycerol + yeast extract) and divalent ions further improved the endospore yield to 5.2x108 spores/mL (at 12 h).
Language EN
URL Website http://tsb2019.com/
Website title The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019)
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บรรณานุกรม

Akarawit Jenjitwanich และผู้แต่งคนอื่นๆ. (2019) Upscaling of RS87 endospore production process - from flask to semi-industrial scale cultivation. The Thai Society for Biotechnology:ม.ป.ท.
Akarawit Jenjitwanich และผู้แต่งคนอื่นๆ. 2019. Upscaling of RS87 endospore production process - from flask to semi-industrial scale cultivation. ม.ป.ท.:The Thai Society for Biotechnology;
Akarawit Jenjitwanich และผู้แต่งคนอื่นๆ. Upscaling of RS87 endospore production process - from flask to semi-industrial scale cultivation. ม.ป.ท.:The Thai Society for Biotechnology, 2019. Print.