รหัสดีโอไอ 10.14455/TSB.res.2019.50
Title Screening and Isolation of Stevioside and Rebaudioside A Degrading Bacteria from Forest Soil
Creator 1. Puey Ounjai
2. Surang Chankhamhaengdecha
Contributor 1. Tanatchakorn Asawasriworanan
2. Phurt Harnvoravongchai
3. Jamorn Samana
Publisher The Thai Society for Biotechnology
Publication Year 2019
Keyword Rebaudioside A ,Steviol Glycosides ,Steviol Glycosides Degrading Bacteria ,Stevioside
Abstract Stevioside and rebaudioside A are the two major steviol glycosides found in Stevia rebaudiana leaves. These glycosides have been widely utilized as natural sugar substitutes in food and beverage industries. Unfortunately, the steviol glycosides quantification are still relied on laborious techniques and expensive analytical instruments, such as mass spectrometry and highperformance liquid chromatography. These analytical techniques are not only time-consuming but also requiring skilled technicians. Previously, an established enzymatic method was developed as a more convenient and inexpensive alternative assay to evaluate stevioside quantity in plant specimen. This study was aimed to screen and identify bacteria with enzymatic capabilities to degrade stevioside and rebaudioside A from soil. The soil samples were collected from the deciduous forests in Nan province, Thailand. A total of 108 bacterial strains were isolated, using minimal salt medium supplemented with stevia leave extract as a sole carbon source. The stevioside and rebaudioside A degradation abilities of each bacterial isolate were investigated using thin-layer chromatography (TLC). Stevioside degrading activity was observed in 3 bacterial isolates. One of them appears to be able to degrade both substrates. The identification based on the sequence of 16S rRNA gene suggested that the bacterial isolates belong to genus Burkholderia, Brevibacillus and Bacillus respectively. Whole genome sequencing of the bacterial isolate capable of degrading both stevioside and rebaudioside A was further performed. The candidate genes responsible for the glycoside degrading activity were preliminarily annotated. While there were a few previous reports of steviol glycosides degrading bacteria, the enzymatic activity reported here appeared to be unique. The glycoside degrading enzymatic activity revealed in this work could be fruitful for broadening the applicability of the enzymatic approach to determine the content of both stevioside and rebaudioside A in Stevia samples.
Language EN
URL Website http://tsb2019.com/
Website title The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019)
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บรรณานุกรม

Puey Ounjai และผู้แต่งคนอื่นๆ. (2019) Screening and Isolation of Stevioside and Rebaudioside A Degrading Bacteria from Forest Soil. The Thai Society for Biotechnology:ม.ป.ท.
Puey Ounjai และผู้แต่งคนอื่นๆ. 2019. Screening and Isolation of Stevioside and Rebaudioside A Degrading Bacteria from Forest Soil. ม.ป.ท.:The Thai Society for Biotechnology;
Puey Ounjai และผู้แต่งคนอื่นๆ. Screening and Isolation of Stevioside and Rebaudioside A Degrading Bacteria from Forest Soil. ม.ป.ท.:The Thai Society for Biotechnology, 2019. Print.