รหัสดีโอไอ 10.14455/TSB.res.2019.49
Title Roles of Histone Deacetylases in Orbital Fibroblasts from Graves' Ophthalmopathy Patients
Creator Sita Virakul
Contributor 1. Supanuch Ekronarongchai
2. Tanapat Palaga
3. Preamjit Saonanon
4. Vannakorn Pruksakorn
5. Willem A. Dik
Publisher The Thai Society for Biotechnology
Publication Year 2019
Keyword Graves' Ophthalmopathy ,Orbital Fibroblasts ,Platelet-Derived Growth Factor-BB ,Histone Deacetylases
Abstract Graves' ophthalmopathy (GO) is an autoimmune thyroid eye disease which mainly causes eyelid retraction and proptosis. Orbital fibroblasts play an important role in GO pathogenesis by increasing cells proliferation, cytokines and hyaluronan production and adipogenesis. Nevertheless, current GO treatments remain limited and novel treatments are needed. Histone deacetylases (HDACs) are enzymes that remove acetyl group from histone tails which plays a role in the regulation of gene expression. HDACs are involved in the pathogenesis of various diseases, including cancer and autoimmune diseases. Therefore, treatment with HDAC inhibitors ( HDACis) was conducted. Currently, the roles of HDACs in orbital fibroblasts from GO remain unidentified. Hence, the aim of this study was to investigate the roles of HDACs in PDGF-BB-induced orbital fibroblast activation using isoformselective HDACis. The results showed that prophylactic effect of HDAC3i (RGFP966), HDAC4i (Tasquinimod) and HDAC4/5i (LMK235) significantly decreased PDGF- BB- induced cell proliferation. In addition, HDAC3i, HDAC4i and HDAC4/5i significantly reduced PDGF-BB-induced hyaluronan production. Unexpectedly, prophylactic effect of HDAC1i ( Quisinostat) , HDAC3i and HDAC4i significantly enhanced PDGF- BB- induced IL- 6 production, while IL-8 production was not affected by HDACis. The level of HDAC3 and HDAC4 expressions increased in PDGF-BB-stimulated orbital fibroblasts. Thus, the data from this study suggested that aberrant HDAC4 levels are involved in PDGF-BB-induced orbital fibroblasts activation.
Language EN
URL Website http://tsb2019.com/
Website title The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019)
ดิจิตอลไฟล์ Digital File

บรรณานุกรม

Sita Virakul และผู้แต่งคนอื่นๆ. (2019) Roles of Histone Deacetylases in Orbital Fibroblasts from Graves' Ophthalmopathy Patients. The Thai Society for Biotechnology:ม.ป.ท.
Sita Virakul และผู้แต่งคนอื่นๆ. 2019. Roles of Histone Deacetylases in Orbital Fibroblasts from Graves' Ophthalmopathy Patients. ม.ป.ท.:The Thai Society for Biotechnology;
Sita Virakul และผู้แต่งคนอื่นๆ. Roles of Histone Deacetylases in Orbital Fibroblasts from Graves' Ophthalmopathy Patients. ม.ป.ท.:The Thai Society for Biotechnology, 2019. Print.