|Title||Invertase Production from Saccharomyces cerevisiae DB-KKU-Y-53 Using Low Cost Medium|
|Contributor||1. Kankanok Charoenpunthuwong
2. Chanika Somsila
|Publisher||The Thai Society for Biotechnology|
|Keyword||Invertase Production ,Saccharomyces cerevisiae ,Mineral Salt Medium ,Response Surface Methodology ,Central Composite Design|
|Abstract||1. Invertase (?-fructofuranosidase
2. EC 22.214.171.124) catalyzes the hydrolysis of sucrose into glucose and fructose. Since these invert sugars have desirable properties such as a source of instant energy, high osmotic pressure and solubility, they are widely used in food, pharmaceutical, cosmetic, medicine and beverage industries. Saccharomyces cerevisiae, a non-pathogenic microorganism, is easily to cultivate and handle during enzyme production. This yeast strain is normally used as the main source of invertase for food industry. In this study, production of invertase from S. cerevisiae DB-KKUY53 using low cost medium has been investigated. To achieve the optimal composition of medium for invertase production, response surface methodology was employed. Screening of the significant variables for enzyme production was conducted using Plackett-Burman Design. The results suggested that concentrations of ammonium sulfate, magnesium sulfate and sucrose were found to be the statistically significant variables for invertase production (p < 0.05). Then, the optimal composition of medium was studied through central composite design. The results indicated that the specific activity of invertase increased to 56.70% (5.14 U/mg) when this yeast was cultivated with the medium containing ammonium sulfate concentration of 3.5 g/l, magnesium sulfate concentration of 1.0 g/l and sucrose concentration of 13.2 g/l, respectively. Furthermore, statistical analysis implied that the mathematical model was statistically significant (p < 0.01). The predicted enzyme activities were in agreement with the experimental results and the difference was less than 10%.
|Website title||The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019)|