รหัสดีโอไอ 10.14455/TSB.res.2019.35
Title Influence of Different Gene Integration Strategies on Transcriptional Silencing at rDNA Gene in Saccharomyces cerevisiae
Creator Chulee Yompakdee
Contributor Pirun Mikled1
Publisher The Thai Society for Biotechnology
Publication Year 2019
Keyword Heterochromatin ,Gene Integration ,rDNA Silencing ,Saccharomyces cerevisiae
Abstract Chromatin silencing at ribosomal DNA (rDNA silencing) is the crucial phenomenon for prevention of homologous recombination of repetitive rDNA sequences. In the previous study, rDNA silencing was shown to play a role in preservation of stability of rDNA (RDN1) locus contributing to enhance yeast lifespan. The silencing efficiency of rDNA locus can be detected by integration of reporter gene into rDNA locus. However, the proper strategy for integration of a reporter gene into rDNA locus has been controversial. This study showed that different type of gene integration strategies, gene insertion and gene replacement, affect silencing capability at rDNA locus of yeast Saccharomyces cerevisiae AS14. A reporter gene, GIN11M86 growthinhibitory sequence under the control of galactose-inducible promoter, was inserted into external transcribed spacer1 (ETS1) gene and replaced into ETS118S (ETS1 RDN18) gene of rDNA locus. The results showed that ETS1 RDN18- replaced strain exhibited a reduced growth rate significantly in comparison with that of the ETS1-inserted and wild-type strain grown under galactose- induced condition. This suggested that gene insertion is the appropriate integration strategy for assessment of rDNA silencing together with study of the critical factor to silencing at rDNA locus.
Language EN
URL Website http://tsb2019.com/
Website title The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019)
ดิจิตอลไฟล์ Digital File

บรรณานุกรม

Chulee Yompakdee,Pirun Mikled1. (2019) Influence of Different Gene Integration Strategies on Transcriptional Silencing at rDNA Gene in Saccharomyces cerevisiae. The Thai Society for Biotechnology:ม.ป.ท.
Chulee Yompakdee,Pirun Mikled1. 2019. Influence of Different Gene Integration Strategies on Transcriptional Silencing at rDNA Gene in Saccharomyces cerevisiae. ม.ป.ท.:The Thai Society for Biotechnology;
Chulee Yompakdee,Pirun Mikled1. Influence of Different Gene Integration Strategies on Transcriptional Silencing at rDNA Gene in Saccharomyces cerevisiae. ม.ป.ท.:The Thai Society for Biotechnology, 2019. Print.