รหัสดีโอไอ 10.14455/TSB.res.2019.31
Title Identification and Characterization of Defensin-related Genes Challenged with Fungal Pathogen Infection in Cassava
Creator Jarunya Narangajavana
Contributor 1. Pattaraporn Hormhuan
2. Budsakorn Dechapitak
3. Punchapat Sojikul
4. Unchera Viboonjun
Publisher The Thai Society for Biotechnology
Publication Year 2019
Keyword Antimicrobial Peptides ,Cassava ,Cassava Anthracnose Disease ,Defensin ,Phytohormones
Abstract Plant defensin family is one of the cysteine-rich antimicrobial peptides, which play important roles in plant immunity for responding against the invasion of pathogens. They are small cationic peptides which are shown to inhibit growth of a broad range of microorganisms. One of the major disease problems on production of cassava (Manihot esculenta Crantz) is cassava anthracnose disease, causing up to 80% yield loss by fungus Collectotrichum gloeosporiodes f. sp. manihotis (CAD). This research aims to identify and characterize defensin-related genes in cassava genome and investigated expression pattern of defensin genes upon CAD pathogen infection and to study the effect of phytohormone pre-treatment prior to fungal infection. The results of bioinformatics analysis showed that the coding sequences of 5 defensin gene members were identified in cassava genome and phylogenetic tree was constructed to verify each gene member. Also, defense-related response motifs were predicted in their promoter regions. Differential expression profiles of MeDef and MeDef1 . 2 were observed in CAD infection and phytohormone pre-treatment of the highly susceptible (HN) and less susceptible/ more resistant (HB60) cassava cultivars. The strong up-regulation of MeDef was found in HB60, whereas late induction of this gene was observed in HN. MeDef1 . 2 was not expressed. Salicylic acid pre-treatment prior to CAD infection could enhance defensive in HB60. This finding suggested that defensin has a potential function in CAD defense response in cassava, and further study should be useful for cassava improvement program to fungal pathogen resistance.
Language EN
URL Website http://tsb2019.com/
Website title The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019)
ดิจิตอลไฟล์ Digital File

บรรณานุกรม

Jarunya Narangajavana และผู้แต่งคนอื่นๆ. (2019) Identification and Characterization of Defensin-related Genes Challenged with Fungal Pathogen Infection in Cassava. The Thai Society for Biotechnology:ม.ป.ท.
Jarunya Narangajavana และผู้แต่งคนอื่นๆ. 2019. Identification and Characterization of Defensin-related Genes Challenged with Fungal Pathogen Infection in Cassava. ม.ป.ท.:The Thai Society for Biotechnology;
Jarunya Narangajavana และผู้แต่งคนอื่นๆ. Identification and Characterization of Defensin-related Genes Challenged with Fungal Pathogen Infection in Cassava. ม.ป.ท.:The Thai Society for Biotechnology, 2019. Print.