รหัสดีโอไอ 10.14455/TSB.res.2019.27
Title Expression of an Immunogenic Protein derived from Spike Protein of Porcine Epidemic Diarrhea Virus in two E. coli Strains
Creator Yaowaluck Maprang Roshorm
Contributor 1. Sochanwattey Meas
2. Pimploy Rattanaamnuaychai
3. Phenjun Mekvichitsaeng
Publisher The Thai Society for Biotechnology
Publication Year 2019
Keyword E. coli ,PEDV ,Protein Expression ,Spike Protein ,Vaccine
Abstract Escherichia coli is recognized as a fast, easy and inexpensive host system for production of valuable proteins including subunit proteins for vaccination. In this study, we studied whether PEDVSME, a new immunogen designed from spike protein of PEDV, can be produced in E. coli by studying in two strains: BL21(DE3) and BL21(DE3)pLysS. The prokaryotic expression plasmid, pET28b, was generated to contain the PEDVSME gene. Recombinant clone of pET28b.PEDVSME was successfully generated and selected in E. coli DH5?. Two strains of E. coli, BL21(DE3) and BL21(DE3)pLysS, were then transformed with recombinant plasmid pET28b.PEDVSME. Expression of the PEDVSME gene was induced with 1 mM IPTG. Analyzed by SDS-PAGE and Western blot, PEDVSME was not detected in E. coli BL21(DE3) but highly expressed in strain BL21(DE3)pLysS. Importantly, although the PEDVSME protein could be observed in soluble form, majority of the protein was found in insoluble form (inclusion body). This work provides useful information regarding appropriate E. coli host strain for efficient production of the immunogen PEDVSME, bringing a feasibility of using PEDVSME as a subunit vaccine.
Language EN
URL Website http://tsb2019.com/
Website title The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019)
ดิจิตอลไฟล์ Digital File

บรรณานุกรม

Yaowaluck Maprang Roshorm และผู้แต่งคนอื่นๆ. (2019) Expression of an Immunogenic Protein derived from Spike Protein of Porcine Epidemic Diarrhea Virus in two E. coli Strains. The Thai Society for Biotechnology:ม.ป.ท.
Yaowaluck Maprang Roshorm และผู้แต่งคนอื่นๆ. 2019. Expression of an Immunogenic Protein derived from Spike Protein of Porcine Epidemic Diarrhea Virus in two E. coli Strains. ม.ป.ท.:The Thai Society for Biotechnology;
Yaowaluck Maprang Roshorm และผู้แต่งคนอื่นๆ. Expression of an Immunogenic Protein derived from Spike Protein of Porcine Epidemic Diarrhea Virus in two E. coli Strains. ม.ป.ท.:The Thai Society for Biotechnology, 2019. Print.