รหัสดีโอไอ 10.14455/TSB.res.2019.25
Title Elementary Flux Mode Analysis of Streptomyces fradiae Metabolic Network for an Increased Neomycin Yield
Creator Asawin Meechai
Contributor 1. Kamonchanok Sumjaiya
2. Chanya Tonaphong
3. Teeraya Praditseree
4. Naraporn Ampornmaha
Publisher The Thai Society for Biotechnology
Publication Year 2019
Keyword Elementary Flux Modes ,Neomycin ,Streptomyces fradiae
Abstract Neomycin is an antibiotic that can naturally be produced by Streptomyces fradiae. Herein, elementary flux mode analysis is used to identify potential gene targets for improvement of neomycin production yield in S. fradiae. To achieve the goal, a metabolic network of S. fradiae is firstly reconstructed by incorporation of 7 key metabolic pathways including glycolysis, tricarboxylic acid cycle, pentose phosphate, pyruvate oxidation, glutamate synthesis from oxoglutarate, glutamate synthesis from glutamine and neomycin synthesis. The main carbon and nitrogen sources for the production are glucose, glutamine and ammonia. After analyzing the reconstructed network by using METATOOL, 230 elementary modes producing neomycin are obtained. These elementary modes show 10 different theoretical yields, ranging from 0.17 to 1 mole of neomycin produced per mole of glucose uptake. It is observed that the modes providing the highest yield use the glucose to synthesize neomycin without entering the glycolysis pathway. From the analysis, it is suggested that the average yield of neomycin in S. fradiae could be improved by knocking out the genes gdh2, gdhA, ssgdh, GLD, gcd, PTS-GLC-EIIA and crr to decrease the total carbon flux in the glycolysis pathway
Language EN
URL Website http://tsb2019.com/
Website title The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019)
ดิจิตอลไฟล์ Digital File

บรรณานุกรม

Asawin Meechai และผู้แต่งคนอื่นๆ. (2019) Elementary Flux Mode Analysis of Streptomyces fradiae Metabolic Network for an Increased Neomycin Yield. The Thai Society for Biotechnology:ม.ป.ท.
Asawin Meechai และผู้แต่งคนอื่นๆ. 2019. Elementary Flux Mode Analysis of Streptomyces fradiae Metabolic Network for an Increased Neomycin Yield. ม.ป.ท.:The Thai Society for Biotechnology;
Asawin Meechai และผู้แต่งคนอื่นๆ. Elementary Flux Mode Analysis of Streptomyces fradiae Metabolic Network for an Increased Neomycin Yield. ม.ป.ท.:The Thai Society for Biotechnology, 2019. Print.