รหัสดีโอไอ 10.14455/TSB.res.2019.24
Title Effects of the Flavonoid Luteolin on Oxidative Stress in Parkinson's Disease Model
Creator Pilaiwanwadee Hutamekalin
Contributor 1. Ratchaneekorn Reudhabibadh
2. Nidanut Champoochana
3. Nipaporn Konthapakdee
4. Pennapa Chonpathompikunlert
Publisher The Thai Society for Biotechnology
Publication Year 2019
Keyword Luteolin ,MPP+ ,Oxidative Stress ,ROS ,SH-SY5Y
Abstract Luteolin is one of the flavonoids compound of numerous traditional medicines. Luteolin has been reported to provide protective effects against apoptosis and oxidative stress. Oxidative stress plays an important role in physiology and pathological conditions. Increasing evidences indicate that the generation of reactive oxygen species (ROS) contributes to oxidative stress, which plays a crucial role in neurodegenerative diseases, including Parkinson's disease ( PD) . Previous studies demonstrated that neurotoxins induced oxidative stress resulted in cell death. Among them, 1- Methyl4phenylpyridinium ion ( MPP+ ) has been widely used to study the role of oxidative stress, leading to cell death in PD. Therefore, optimization of ROS production is considered to be target to prevent PD. However, the effect of luteolin on PD via ROS generation is still obscure. In this study, human neuroblastoma SH-SY5Y cells were pre-treated with luteolin and NAC for 1 h, following treatment with 100 ?M MPP+. After treatment, cell viability was measured using MTT assay. Apoptotic cell death was observed by Hoechst 33342 staining . Intracellular ROS was measured using DCFH-DA. The results demonstrated that luteolin inhibited MPP+-induced cell death. In addition, luteolin and NAC reduced ROS generation in MPP+-treated SH-SY5Y cells. This study provides evidence that luteolin has a potent neuroprotective effect against MPP+-induced cell death via the reduction of oxidative stress.
Language EN
URL Website http://tsb2019.com/
Website title The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019)
ดิจิตอลไฟล์ Digital File

บรรณานุกรม

Pilaiwanwadee Hutamekalin และผู้แต่งคนอื่นๆ. (2019) Effects of the Flavonoid Luteolin on Oxidative Stress in Parkinson's Disease Model. The Thai Society for Biotechnology:ม.ป.ท.
Pilaiwanwadee Hutamekalin และผู้แต่งคนอื่นๆ. 2019. Effects of the Flavonoid Luteolin on Oxidative Stress in Parkinson's Disease Model. ม.ป.ท.:The Thai Society for Biotechnology;
Pilaiwanwadee Hutamekalin และผู้แต่งคนอื่นๆ. Effects of the Flavonoid Luteolin on Oxidative Stress in Parkinson's Disease Model. ม.ป.ท.:The Thai Society for Biotechnology, 2019. Print.