รหัสดีโอไอ 10.14455/TSB.res.2019.23
Title Effect of Metformin on Reducing Self-renewal and Proliferative Ability in Neuroblastoma SH-SY5Y Cells by Induction of Cell Differentiation
Creator Pilaiwanwadee Hutamekalin
Contributor 1. Thunwa Binlateh
2. Supita Tanasawet
Publisher The Thai Society for Biotechnology
Publication Year 2019
Keyword Neuroblastoma ,Metformin ,Self-Renewal ,Differentiation ,Proliferation
Abstract Neuroblastoma (NB), the most common extracranial solid tumor, is challenged by high rate of relapse and drug-resistance. These may due to a presence of cancer stem cell (CSC) subpopulation in NB which relates to high capacity of self-renewal and differentiation. Recently, it has been believed that the promotion of cancer cells differentiation can irreversible change in the cancer phenotypes, particularly in loss of self-renewal. Consequently, drugs that trigger differentiation in cancers have currently being explored. In the present study, we showed the role of metformin in promoting differentiation and its effect on self-renewal capacity. The results revealed that metformin can promote cell differentiation with increasing neurite length and neuronal markers, ?-tubulin III and MAP-2. Furthermore, the ability of cell proliferation at day 2 and 3 after metformin-mediated cell differentiation was significantly diminished. Likewise, self- renewal capacity was decreased in metforminpromoted cell differentiation as determined by significant reduction of spheroid number and size. The percentage of cell in G0/ G1 phase was markedly increased in metformin-treated cells compared to control untreated cells. Together, Metformin has a potential role to promote differentiation and reduces self-renewal capacity and ability of cell proliferation after promoting differentiation.
Language EN
URL Website http://tsb2019.com/
Website title The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019)
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บรรณานุกรม

Pilaiwanwadee Hutamekalin และผู้แต่งคนอื่นๆ. (2019) Effect of Metformin on Reducing Self-renewal and Proliferative Ability in Neuroblastoma SH-SY5Y Cells by Induction of Cell Differentiation. The Thai Society for Biotechnology:ม.ป.ท.
Pilaiwanwadee Hutamekalin และผู้แต่งคนอื่นๆ. 2019. Effect of Metformin on Reducing Self-renewal and Proliferative Ability in Neuroblastoma SH-SY5Y Cells by Induction of Cell Differentiation. ม.ป.ท.:The Thai Society for Biotechnology;
Pilaiwanwadee Hutamekalin และผู้แต่งคนอื่นๆ. Effect of Metformin on Reducing Self-renewal and Proliferative Ability in Neuroblastoma SH-SY5Y Cells by Induction of Cell Differentiation. ม.ป.ท.:The Thai Society for Biotechnology, 2019. Print.