รหัสดีโอไอ 10.14455/TSB.res.2019.19
Title Development of Enzymatic Assay for Adenosine Quantification in Cordyceps militaris Extract
Creator Panwajee Payongsri
Contributor 1. Yanisa Suebsuk
2. Chananya Tansathaporn
Publisher The Thai Society for Biotechnology
Publication Year 2019
Keyword Adenosine ,Adenosine Deaminase ,Cordycepin ,Cordyceps militaris ,Extraction
Abstract Cordyceps militaris is a substituent of high price Chinese traditional medicine, Ophiocordyceps sinensis. This fungus is widely cultivated and consumed for health benefits in Thailand. Currently, the quality can be evaluated by the concentration of adenosine and cordycapin which relies on HPLC. This study proposed a simple enzymatic method for quantifying adenosine in C. militaris extract. In this research, C. militaris samples were extracted by hot water extraction (80?C, 3h). Recombinant eADA (Escherichia coli adenosine deaminase) adenosine converted to inosine and this was continuously observed by spectrophotometer at 265 nm. The total change in OD265 can be used to quantify adenosine in the extract in a good agreement with quantification by HPLC. During this research, it was observed that high temperature extraction led to adenosine degradation and up to 30% reduction could be observed after 3-hour extraction. However, this phenomenon was not observed with cordycepin.
Language EN
URL Website http://tsb2019.com/
Website title The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019)
ดิจิตอลไฟล์ Digital File

บรรณานุกรม

Panwajee Payongsri และผู้แต่งคนอื่นๆ. (2019) Development of Enzymatic Assay for Adenosine Quantification in Cordyceps militaris Extract. The Thai Society for Biotechnology:ม.ป.ท.
Panwajee Payongsri และผู้แต่งคนอื่นๆ. 2019. Development of Enzymatic Assay for Adenosine Quantification in Cordyceps militaris Extract. ม.ป.ท.:The Thai Society for Biotechnology;
Panwajee Payongsri และผู้แต่งคนอื่นๆ. Development of Enzymatic Assay for Adenosine Quantification in Cordyceps militaris Extract. ม.ป.ท.:The Thai Society for Biotechnology, 2019. Print.