รหัสดีโอไอ 10.14455/TSB.res.2019.18
Title Design, Generation and Protein Expression of the EpitopeBased PEDV (Porcine Epidemic Diarrhea Virus) Vaccine
Creator Yaowaluck Maprang Roshorm
Contributor 1. Kanokporn Polyiam
2. Marasri Ruengjitchatchawalya
3. Teerapan Laomettachit
4. Kanokwan Poomputsa
5. Phenjun Mekvichitsaeng
Publisher The Thai Society for Biotechnology
Publication Year 2019
Keyword PEDV ,Epitope-Based Vaccine ,DNA Vaccine ,Adenovirus ,E . coli
Abstract 1. Porcine Epidemic Diarrhea (PED) continues to cause huge economic losses in swine industry worldwide. Porcine Epidemic Diarrhea Virus (PEDV), the causative agent of PED, exhibits highly genetic variation
2. thus, a universal vaccine is desirable. Our main goal is to develop a universal vaccine using multi-epitope strategy. In this study, we designed, generated and studied the expression of the PEDV multi-epitope vaccines. Two new vaccine antigens, epiS(MHC) and epiS(CTL), were designed to contain CD8 T cell epitopes obtained from in silico prediction of the PEDV spike protein (S). The vaccines were then developed using 3 different vaccine systems. In HEK293A, the antigens produced by DNA vaccines and adenovirus-based vaccines exhibited only small amount of the protein. When expressed in E . coli expression system, only the epiS(MHC) protein displayed full-length and degraded forms of the protein when it is detected by Western blot. The results of protein expression by 3 different vaccine platforms suggest that epitope-based vaccine can be very unstable and susceptible to degradation and further modification is important to help improve stability and functionality of the vaccines.
Language EN
URL Website http://tsb2019.com/
Website title The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019)
ดิจิตอลไฟล์ Digital File

บรรณานุกรม

Yaowaluck Maprang Roshorm และผู้แต่งคนอื่นๆ. (2019) Design, Generation and Protein Expression of the EpitopeBased PEDV (Porcine Epidemic Diarrhea Virus) Vaccine. The Thai Society for Biotechnology:ม.ป.ท.
Yaowaluck Maprang Roshorm และผู้แต่งคนอื่นๆ. 2019. Design, Generation and Protein Expression of the EpitopeBased PEDV (Porcine Epidemic Diarrhea Virus) Vaccine. ม.ป.ท.:The Thai Society for Biotechnology;
Yaowaluck Maprang Roshorm และผู้แต่งคนอื่นๆ. Design, Generation and Protein Expression of the EpitopeBased PEDV (Porcine Epidemic Diarrhea Virus) Vaccine. ม.ป.ท.:The Thai Society for Biotechnology, 2019. Print.