Evaluation on Phytochemical Constituents and Antioxidant Activities of Various Formula from Ko-Klan Remedies by Aqueous Infusion Preparation Method
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Title Evaluation on Phytochemical Constituents and Antioxidant Activities of Various Formula from Ko-Klan Remedies by Aqueous Infusion Preparation Method
Creator Khwanchnok Maitnork
Contributor Ampa Konsue
Publisher Faculty of Technology, Mahasarakham University
Publication Year 2562
Keyword Ko-Klan, Phenol, Flavonoids, Antioxidantion, Infusion, DPPH, (ABTS+)
Abstract 1. Ko-Klan remedy is Thai folklore medicine appear on list of Thai herbal medicinal products which have been widely used to relief of muscle pain. The remedy were separated to 3 recipes
2. first, Mallotus repandus: Rhinacanthus nasutus: Elephantopus scaber: Aegle marmelos
3. 1: 1:1:1 (w:w:w:w), second, Mallotus repandus: Rhinacanthus nasutus: Elephantopus scaber: Aegle marmelos
4. 5:2.5:1.5:1.5 (w:w:w:w), third, Mallotus repandus: Rhinacanthus nasutus: Elephantopus scabe: Ceasalpinia sappan: Cryptolepis buchanani: Piper interruptum
5. 2:1:1:2:2:2 (w:w:w:w). Aims of this study were evaluated to total phenolic (TPC) and flavonoids (TFC) content, and antioxidant activities of various formulas from Ko-Klan remedy extracts by water infusion preparation. The TPC and TFC were determined to chemical composition. The antioxidations were tested using by 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging, and 2,2 -azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS+) assay. The results showed that, the 1st remedy were higher contents of TPC and TFC (12.7927?0.0762 mgQE/gExt and 40.7925? 0.5372 mgGE/gExt), the 2nd remedy was highest antioxidant activity by ABTS assay (IC50 = 0.0022? 0.00002 mg/mL), the 3rd remedy were highest free radical scavenging by DPPH assay (IC50 = 0.0122? 0.00005 mg/mL). The finding studies suggest that all of the remedy were antioxidation higher than Ascorbic acid and Trolox as standard substances. Further study, some chemical compound(s) and pharmaceutical activity were clarified to medicinal plant use on health promotion.
Language EN
URL Website https://techno2.msu.ac.th/icofab2019/abstract-collection-and-proceedings/
Website title The 6th International Conference on Food Agriculture & Biotechnology(ICoFAB 2019)
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