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Phytochemical Screening, Antioxidant, and alpha-Glucosidase Inhibitory Activities of Different Solvent Extracts from Leersia hexandra and Elephantopus scaber |
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| รหัสดีโอไอ | |
| Title | Phytochemical Screening, Antioxidant, and alpha-Glucosidase Inhibitory Activities of Different Solvent Extracts from Leersia hexandra and Elephantopus scaber |
| Creator | Ampa Konsue |
| Contributor | Saithong Sombutphoothorn1 |
| Publisher | Faculty of Technology, Mahasarakham University |
| Publication Year | 2562 |
| Keyword | Phytochemical screening, Phenol, Flavonoids, Antioxidant, alpha-glucosidase |
| Abstract | 1. The aim of this research was to determine on phytochemical screening, antioxidant and alpha glucosidase inhibitory activities of Leersia hexandra and Elephantopus scaber by using different solvent extractions. Both plants (1:1 2. w:w) of recipe were extracted using by different solvents including aqueous (ALE) and 80% ethanol (ELE). Phytochemical screening was determined on total phenolic (TPC) and flavonoid (TFC) contents in plants. The antioxidant activities were determined by, 2,2-diphenyl-1- picrylhydrazy (DPPH) radical scavenging and 2,2-azinobis(3-ethylbenzothiazoline-6-sulphonate) (ABTS+ ) assay. The -glucosidase inhibitory assay was evaluated on glucose transferase mechanism. This experimental study found that the recipe showed high among of total phenolic and flavonoid contents especially ELE (40.024?0.952 mgGE/gExt and 0.072?0.001 mgQE/gExt,). The ELE (IC50 = 0.082?0.0025 mg/mL) was significantly exerted on free radical scavenging activity higher than ALE (IC50 = 0.122?0.0033 mg/mL). ABTS+ radical scavenging activity, the ELE (IC50 = 0.0048?0.00018 mg/mL) was significantly stronger than Trolox (IC50 = 0.0086?0.00063 mg/mL), known as standard substances. ?-glucosidase inhibitory activity, ALE (IC50 = 0.022?0.001 mg/mL) and ELE (IC50 = 0.098?0.002 mg/mL) were significantly more effect in inhibited ?-glucosidase enzyme than acarbose (IC50 =1.054?0.113 mg/mL), known anti-diabetic drug. The recipe has been having the phenolic and flavonoid contents which chemical substances were known as anti-oxidation and anti-diabetic property, Pharmaceutical activities were showed on antioxidant and ?-glucosidase inhibitory activities. Further, also chemical compositions, major active compound(s) and in vivo were clarified in next study. |
| Language | EN |
| URL Website | https://techno2.msu.ac.th/icofab2019/abstract-collection-and-proceedings/ |
| Website title | The 6th International Conference on Food Agriculture & Biotechnology(ICoFAB 2019) |
