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Comparative Study of Proteome Pattern of Kluai Ta Nee and Kluai Nam Wa Leaf Proteins |
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| รหัสดีโอไอ | |
| Title | Comparative Study of Proteome Pattern of Kluai Ta Nee and Kluai Nam Wa Leaf Proteins |
| Creator | Bung-on Prajanban |
| Contributor | Wichuda Jankangram, Nisachon Jangpromma |
| Publisher | Faculty of Technology, Mahasarakham University |
| Publication Year | 2562 |
| Keyword | 2D-PAGE, Kluai Ta Nee, Kluai Nam Wa, banana leaves |
| Abstract | This study compared the protein profiles in leaves of diploid and triploid banana to identify the differentially accumulated proteins unique to each type of banana. Protein samples were analyzed by 2DPAGE using IPG strip pH3-10. Fifty and 44 protein spots were detected in leaves of Musa acumimata (BB group) "Kluai Ta Nee" and Musa Xparadisiaca (ABB group) "Kluai Nam Wa", respectively. Many spots of leaf proteins were corresponding to leaf proteins of Musa acuminate Colla (banana) reported previously such as ribulose-1,5-bisphosphate carboxylase, oxygen-evolving enhancer protein and superoxide dismutase. Ribulose-1,5-bisphosphate carboxylase, oxygen-evolving enhancer protein and superoxide dismutase in Kluai Ta Nee leaves had pIs and number of isoforms different from those in Kluai Nam Wa leaves. Three isoforms of endochitinase were found in Kluai Ta Nee leaves only. The major protein found in Kluai Ta Nee leaves, which had higher intensity than that in Kluai Nam Wa leaves, was identified as ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit. A low quality protein: oxygen-evolving enhancer protein 1, chloroplastic-like, found in Kluai Nam Wa leaves had higher intensity than that in Kluai Ta Nee leaves. The polymorphism of these proteins observed between leaves of Kluai Ta Nee and Kluai Nam Wa would be possibly due to different gene expressions in each banana type and may result in different functionalities of the proteins. |
| Language | EN |
| URL Website | https://techno2.msu.ac.th/icofab2019/abstract-collection-and-proceedings/ |
| Website title | The 6th International Conference on Food Agriculture & Biotechnology(ICoFAB 2019) |
