Genetic Variation among Thai Dugong (Dugong dugon) Populations from Cytochrome C Oxidase Subunit 1 DNA Sequence Data
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Title Genetic Variation among Thai Dugong (Dugong dugon) Populations from Cytochrome C Oxidase Subunit 1 DNA Sequence Data
Creator Nattapong Srisamoot
Contributor Kongkiat Kittiwatanawong
Publisher Faculty of Technology, Mahasarakham University
Publication Year 2562
Keyword Dugong, Dugong dugon, Genetic variation, Cytochrome C oxidase, COI
Abstract Dugong (Dugong dugon) was classified as vulnerable and currently considered rare over most of this range. The population of dugong in Thailand was found in fragmented habitats along the Thai coast. Genetic studies were used in this study to determine the variation of cytochrome c oxidase subunit 1 (COI) DNA sequence of ten dugong samplesfrom Krabi, Phuket, Trang and Satun Province. The DNA extraction result showed that the samples stored in ethanol can maintain the integrity of the genetic material for a long time. The length of COI sequence was 794 to 859 bp and the average length was 823.4 bp. All COI sequences in this study were identical to GenBank accession numbers AY075116.1 which have an average of 91.34%. The lowest genetic distance (0.005) was between dugong 3 and dugong 4, while the highest (0.163) was between dugong 6 and dugong 10 with an average of 0.070. The low genetic distance demonstrated that the gene flow between the dugong population in each area still occurred. The dendrogram constructed from COI sequence comparisons using the Maximum Likelihood method based on the TamuraNei model divided ten dugong samples into three subgroups. The major group comprised of seven dugong samples which were collected around Koh Libong and the coastal areas of Trang Province. The clustering corresponds to the area with the largest and abundant seagrass
Language EN
URL Website https://techno2.msu.ac.th/icofab2019/abstract-collection-and-proceedings/
Website title The 6th International Conference on Food Agriculture & Biotechnology(ICoFAB 2019)
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