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Isolation and Identification of Lipase-Producing Bacteria from Soil in Nasinuan Forest, Kantarawichai District, Mahasarakham Province |
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| รหัสดีโอไอ | |
| Title | Isolation and Identification of Lipase-Producing Bacteria from Soil in Nasinuan Forest, Kantarawichai District, Mahasarakham Province |
| Creator | Manatchanok Yotchaisarn |
| Contributor | Sirirat Deeseenthum, Kedsukon Maneewan, Vijitra Luang-In |
| Publisher | Faculty of Technology, Mahasarakham University |
| Publication Year | 2562 |
| Keyword | Bacteria, Soil, Lipase enzyme, Industry, 16S rRNA gene |
| Abstract | 1. Nasinuan Forest was located at Kantarawichai District, Mahasarakham Province Thailand. This area has been considered to be quite saline 2. however contains a variety of bacteria that may be able to produce industrial enzymes. Lipase is one of the industrial enzymes which play an important role in industry in Thailand such as detergent industry, food, fat and oil industries and cosmetics. This study aimed to identify lipase-producing bacteria from soil in Nasinuan Forest and determine the optimum conditions for lipase production and lipase activity. The results showed that 35 isolates were positive for lipase after being tested on tributyrin agar. The top five isolates with the highest halo : colony ratios included Bacillus subtilis 1.3LP2 > Brevibacillus brevis 2.1LP4 > Micrococcus sp. 3.2LP3 > Bacillus thuringiensis 1.1LP3 > Staphylococcus sp. 1.4LP4, respectively. The results of gram staining found that four isolates were grampositive with rod-shaped and only gram-positive with cocci-shaped was found as Staphylococcus sp. 1.4LP4. These lipase-producing bacteria were closest to Bacillus spp., Staphylococcus sp., Brevibacillus sp. and Micrococcus sp. using 16S rRNA gene analysis whose from Italy, India, China, Egypt and Brazil. The optimum conditions for the highest lipase activity by Bacillus subtilis 1.3LP2 are pH 7.0 at 55 ?C with the lipase activity of 160.92 ? 0.91 U/mL. This is the first report of lipase-producing bacteria isolated from soil in Nasinuan forest, Mahasarakham, Thailand. These bacterial enzymes can be applied in various industries in Thailand such as detergent industry, food, fat and oil industries and cosmetics. |
| Language | EN |
| URL Website | https://techno2.msu.ac.th/icofab2019/abstract-collection-and-proceedings/ |
| Website title | The 6th International Conference on Food Agriculture & Biotechnology(ICoFAB 2019) |
