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Locust Bean Gum Hydrolysis for Mannooligosaccharide (MOS) Production Using Bacillus methylotrophicus KS1 |
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| รหัสดีโอไอ | |
| Title | Locust Bean Gum Hydrolysis for Mannooligosaccharide (MOS) Production Using Bacillus methylotrophicus KS1 |
| Creator | Sunchai Phiwphech |
| Contributor | Vijitra Luang-In, Sirirat Deeseenthum, Surachai Rattanasuk |
| Publisher | Faculty of Technology, Mahasarakham University |
| Publication Year | 2562 |
| Keyword | Bacillus methylotrophicus KS1, Locust bean gum, Mannanase, Mannooligosaccharide, HPLC |
| Abstract | Locust bean gum is a source of galactomannan that can be used as substrate for mannooligosaccharide production with prebiotic potential through mannanase-catalyzed hydrolysis. The aim of this research was to hydrolyze the locust bean gum using Bacillus methylotrophicus KS1 isolated from soil collected from Roi Et, Thailand. Locust bean gum hydrolysis conditions were performed using 1% Bacillus methylotrophicus KS1 inoculum (108 CFU/ml) in nutrient broth containing 1% locust bean gum in 500 ml Erlenmeyer flask and incubated at 37 oC and 150 rpm for 24 h. Five milliliters of cultures were collected at the specific time intervals for mannanase activity determination. The result indicated that the highest mannanase activity was found at 18 h with 14.10 U/ml. Mannooligosaccharide composition of locust bean gum hydrolysis was identified using High Performance Liquid Chromatography method. The result showed that the produced mannooligosaccharide consisted of mannotriose, mannotetraose and mannohexose. These may have prebiotic attributes that will be investigated in the future. |
| Language | EN |
| URL Website | https://techno2.msu.ac.th/icofab2019/abstract-collection-and-proceedings/ |
| Website title | The 6th International Conference on Food Agriculture & Biotechnology(ICoFAB 2019) |
